{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T12:16:15Z","timestamp":1783685775817,"version":"3.55.0"},"reference-count":46,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,5,8]],"date-time":"2023-05-08T00:00:00Z","timestamp":1683504000000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Osteoarthritis is a degenerative knee joint disease featured with articular cartilage degeneration and inflammation. Alisol A 24\u2010acetate (ALA\u201024A) is an active triterpene that has antioxidant and anti\u2010inflammatory pharmacological properties. However, its effect and molecular mechanism on osteoarthritis progression have not been reported.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>IL\u20101\u03b2\u2010induced chondrocyte injury model and monosodium iodoacetate (MIA)\u2010induced rat osteoarthritis model were used. The protective effects of ALA\u201024A on osteoarthritis were evaluated by determining cell viability, extracellular matrix (ECM) degradation, inflammatory response and oxidative stress using CCK\u20108 assay, Western blot, ELISA, and DCFH\u2010DA fluorescent probe. The severity and matrix degradation of articular cartilage were assessed by histopathological and immunohistochemical examination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that ALA\u201024A attenuated IL\u20101\u03b2\u2010induced cell viability inhibition Moreover, ALA\u201024A suppressed expression levels of ECM degradation\u2010related genes ADAMTS5 and MMP13, and promoted expression levels of ECM synthesis\u2010related genes Aggrecan and Collagen II. In addition, ALA\u201024A treatment decreased reactive oxygen species (ROS) production and increased antioxidant enzymes (SOD, CAT, and GSH\u2010px) activities, while increased MDA levels. The inflammatory levels of NO, PGE2, TNF\u2010\u03b1, and IL\u20106 were also reduced following treatment with ALA\u201024A. Our data also revealed that ALA\u201024A treatment triggered p\u2010AMPK upregulation and p\u2010mTOR downregulation. In rat osteoarthritis model, ALA\u201024A treatment significantly alleviated the severity and matrix degradation of articular cartilage comparted with model group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our findings suggested a protective role of ALA\u201024A against osteoarthritis by inhibiting ROS and inflammatory response. Furthermore, ALA\u201024A might be a promising therapeutic option for osteoarthritis treatment.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.848","type":"journal-article","created":{"date-parts":[[2023,5,8]],"date-time":"2023-05-08T13:27:24Z","timestamp":1683552444000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Alisol A 24\u2010acetate ameliorates osteoarthritis progression by inhibiting reactive oxygen species and inflammatory response through the AMPK\/mTOR pathway"],"prefix":"10.1002","volume":"11","author":[{"given":"Guosong","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Orthopedics The First Hospital of Putian City Putian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhensen","family":"He","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The First Hospital of Putian City Putian 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