{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T14:42:04Z","timestamp":1792593724088,"version":"4.5.0"},"reference-count":41,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,5,16]],"date-time":"2023-05-16T00:00:00Z","timestamp":1684195200000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772399"],"award-info":[{"award-number":["81772399"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Excessive oxidative stress has been accepted as one of the critical factors for intervertebral disc degeneration (IDD), which is associated with low back pain (LBP). Fisetin (Fis) is a bioactive flavonoid that possesses strong bioactive activity. In present study, we aimed to illuminate the role of Fis on nucleus pulposus mesenchymal stem cells (NPMSCs).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>NPMSCs were isolated and cultured from rat NP tissues and identified by flow cytometry and multilinear differentiation. The cytotoxicity of Fis, EX\u2010527, and hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) on NPMSCs was validated using Cell Counting Kit\u20108 tests. Cell apoptosis was tested by flow cytometry and TUNEL assay. Inflammatory mediators were assessed by Elisa tests, RT\u2010PCR. Extracellular matrix (ECM) metabolism was measured by Western blot analysis and RT\u2010qPCR. The expression of the SIRT1 was evaluated by Western blot analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>NPMSCs were successfully isolated and cultured from rat NP tissues, and it has been identified by flow cytometry and multilinear differentiation. The results showed that Fis attenuated H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced apoptosis, inflammation, and ECM degradation of NPMSCs. Moreover, the above protective effects of Fis can be inhibited by EX\u2010527, a unique SIRT1 inhibitor, indicating that SIRT1 may involve in the mechanism of Fis in protecting NPMSCs from oxidative stress.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>As a natural compound with little cytotoxicity on NPMSCs, Fis alleviate H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced apoptosis, inflammation, and ECM degradation by suppressing oxidative stress, this finding may add the theoretical basis for research on new treatment of IDD based on NPMSCs.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.865","type":"journal-article","created":{"date-parts":[[2023,5,16]],"date-time":"2023-05-16T11:36:08Z","timestamp":1684236968000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Fisetin regulates the biological effects of rat nucleus pulposus mesenchymal stem cells under oxidative stress by sirtuin\u20101 pathway"],"prefix":"10.1002","volume":"11","author":[{"given":"Qing","family":"Zhou","sequence":"first","affiliation":[{"name":"Navy Clinical College Anhui Medical University Hefei Anhui China"},{"name":"The Fifth School of Clinical Medicine Anhui Medical University Hefei Anhui China"},{"name":"Department of Orthopedic Surgery The Sixth Medical Center of PLA General Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery The Sixth Medical Center of PLA General Hospital Beijing China"},{"name":"The Second School of Clinical Medicine Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anwu","family":"Xuan","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery The Sixth Medical Center of PLA General Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junyou","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery The Sixth Medical Center of PLA General Hospital Beijing China"},{"name":"The Second School of Clinical Medicine Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenbiao","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery The Sixth Medical Center of PLA General Hospital Beijing China"},{"name":"The Second School of Clinical Medicine Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery The Sixth Medical Center of PLA General Hospital Beijing China"},{"name":"The Second School of Clinical Medicine Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9642-3096","authenticated-orcid":false,"given":"Dike","family":"Ruan","sequence":"additional","affiliation":[{"name":"Navy Clinical College Anhui Medical University Hefei Anhui China"},{"name":"The Fifth School of Clinical Medicine Anhui Medical University Hefei Anhui China"},{"name":"Department of Orthopedic Surgery The Sixth Medical Center of PLA General Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,5,16]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.E.01273"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007632-199612150-00002"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007632-200002150-00016"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/os.13302"},{"key":"e_1_2_11_6_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2017\/5601593","article-title":"ROS: crucial intermediators in the pathogenesis of intervertebral disc degeneration","volume":"2017","author":"Feng C","year":"2017","journal-title":"Oxid Med Cell Longevity"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-022-02804-y"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/stcltm\/szac013"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1159\/000369452"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.4901"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/130.9.2243"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-012-9866-x"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.109772"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1586\/14737167.6.3.359"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00586-016-4513-5"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.23195"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12905-021-01298-1"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.1023877"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41413-020-00125-x"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22073519"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.brs.0000259225.37454.38"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reth.2021.12.001"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2019.07.026"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/5474289"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.3340\/jkns.2013.53.4.207"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.822501"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.3441"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.4252\/wjsc.v12.i12.1603"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-022-04462-9"},{"key":"e_1_2_11_30_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2022\/1949718","article-title":"Natural compounds for SIRT1\u2010mediated oxidative stress and neuroinflammation in stroke: a potential therapeutic target in the future","volume":"2022","author":"Fang C","year":"2022","journal-title":"Oxid Med Cell Longevity"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2020.568861"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.831168"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389450116666150630110529"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2022.108671"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mehy.2021.110757"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11357-012-9474-y"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0000000000001954"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.109592"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.10.094"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.808480"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1080\/13510002.2022.2116551"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X22500938"}],"container-title":["Immunity, Inflammation and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iid3.865","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T18:22:42Z","timestamp":1744395762000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/iid3.865"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5]]},"references-count":41,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,5]]}},"alternative-id":["10.1002\/iid3.865"],"URL":"https:\/\/doi.org\/10.1002\/iid3.865","archive":["Portico"],"relation":{},"ISSN":["2050-4527","2050-4527"],"issn-type":[{"value":"2050-4527","type":"print"},{"value":"2050-4527","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5]]},"assertion":[{"value":"2022-12-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e865"}}