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Current drug therapies inhibit viral replication, slow the progression of liver fibrosis and reduce infectivity, but they rarely remove the covalently sealed circular DNA (cccDNA) of the virus that causes HBV persistence. Alternative treatment strategies, including those based on CRISPR\/cas9 knockout virus gene, can effectively inhibit HBV replication, so it has a good prospect. During chronic infection, some virus gene knockouts based on CRISPR\/cas9 may even lead to cccDNA inactivation. This paper reviews the progress of different HBV CRISPR\/cas9, vectors for delivering to the liver, and the current situation of preclinical and clinical research.<\/jats:p>","DOI":"10.1002\/iid3.866","type":"journal-article","created":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T02:58:44Z","timestamp":1683773924000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["CRISPR\/Cas9 for hepatitis B virus infection treatment"],"prefix":"10.1002","volume":"11","author":[{"given":"Bo","family":"Cai","sequence":"first","affiliation":[{"name":"Center of Medical Genetics Northwest Women's and Children's Hospital  Xi'an Shaanxi PR. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shixue","family":"Chang","sequence":"additional","affiliation":[{"name":"Center for Translational Medicine The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shaanxi PR. 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