{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T06:42:19Z","timestamp":1784097739902,"version":"3.55.0"},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T00:00:00Z","timestamp":1685059200000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Approximately 50% of cases with recurrent spontaneous abortion (RSA) have unexplained etiology. Aberrant expression of transmembrane and ubiquitin\u2010like domain containing 1 (TMUB1) is closely related to a series of diseases, including RSA. However, the function and underlying mechanism of TMUB1 in the occurrence of RSA has not been described.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>TMUB1 expression was detected in the placental villous tissues of 30 women with normal miscarriages and 12 women with RSA. The pregnant mice were injected intraperitoneally with lipopolysaccharide (LPS) to induce abortion. Human chorionic trophoblast cells were treated with LPS. Pathological analysis of placental tissues was performed by hematoxylin and eosin staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>TMUB1 was highly expressed in the placental villous tissues of RSA patients compared to the patients who underwent induced abortions. After LPS administration, the mice exhibited high embryo absorption and pathological alterations, as well as presented an increase in inflammation and apoptosis (the etiology of RSA induction) in placental tissues. Moreover, the upregulated expression of TMUB1 was also found in placental tissues of LPS\u2010induced mice, and further investigation showed that TMUB1 deficiency blocked embryo loss as well as inhibited apoptotic rate and inflammation after LPS activation. Furthermore, we found that the loss of TMUB1 suppressed the phosphorylation of IkappaB kinase (IKK) \u03b1\/\u03b2 and attenuated cytoplasmic\u2010nuclear translocation of nuclear factor\u2010\u03baB (NF\u2010\u03baB) p65 in LPS\u2010induced cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results indicate that TMUB1 may involve in the modulation of apoptosis and NF\u2010\u03baB pathway\u2010mediated inflammation in RSA. Therefore, TMUB1 may develop as a potential biomarker for RSA treatment.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.879","type":"journal-article","created":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T11:29:46Z","timestamp":1685100586000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Inhibition of TMUB1 blocks apoptosis and NF\u2010\u03baB pathway\u2010mediated inflammation in recurrent spontaneous abortion"],"prefix":"10.1002","volume":"11","author":[{"given":"Xiuping","family":"Zhang","sequence":"first","affiliation":[{"name":"Reproductive Medicine Center Children's Hospital of Shanxi and Women Health Center of Shanxi Taiyuan Shanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanjing","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Gynecologic Oncology Tianjin Central Hospital of Gynecology Obstetrics Tianjin 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