{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T19:18:11Z","timestamp":1790363891955,"version":"4.1.0"},"reference-count":67,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,6,14]],"date-time":"2023-06-14T00:00:00Z","timestamp":1686700800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003995","name":"Natural Science Foundation of Anhui Province","doi-asserted-by":"publisher","award":["2208085MH224"],"award-info":[{"award-number":["2208085MH224"]}],"id":[{"id":"10.13039\/501100003995","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81901238"],"award-info":[{"award-number":["81901238"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Melatonin (MEL), an endogenous hormone, has been widely investigated in neurological diseases. Microglia (MG), a resident immunocyte localizing in central nervous system is reported to play important functions in the animal model of temporal lobe epilepsy (TLE). Some evidence showed that MEL influenced activation of MG, but the detailed model of action that MEL plays in remains uncertain.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this study, we established a model of TLE in mice by stereotactic injection of kainic acid (KA). We treated the mice with MEL. Lipopolysaccharide, ROCK2\u2010knockdown (ROCK\u2010KD) and \u2010overexpression (ROCK\u2010OE) of lentivirus\u2010treated cells were used in cell experiments to simulate an in vitro inflammatory model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The results of electrophysiological tests showed that MEL reduced frequency and severity of seizure. The results of behavioral tests indicated MEL improved cognition, learning, and memory ability. Histological evidences demonstrated a significant reduction of neuronal death in the hippocampus. In vivo study showed that MEL changed the polarization status of MG from a proinflammatory M1 phenotype to an anti\u2010inflammatory M2 phenotype by inversely regulating the RhoA\/ROCK signaling pathway. In cytological study, we found that MEL had a significant protective effect in LPS\u2010treated BV\u20102 cells and ROCK\u2010KD cells, while the protective effect of MEL was significantly attenuated in ROCK\u2010OE cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>MEL played an antiepileptic role in the KA\u2010induced TLE modeling mice both in behavioral and histological levels, and changed MG polarization status by regulating the RhoA\/ROCK signaling pathway.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/iid3.900","type":"journal-article","created":{"date-parts":[[2023,6,14]],"date-time":"2023-06-14T09:16:21Z","timestamp":1686734181000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Melatonin regulates microglial polarization to M2 cell via RhoA\/ROCK signaling pathway in epilepsy"],"prefix":"10.1002","volume":"11","author":[{"given":"Pingping","family":"Li","sequence":"first","affiliation":[{"name":"Department of Neurosurgery The First Affiliated Hospital of Anhui Medical University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuefei","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The First Affiliated Hospital of Anhui Medical University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Shan","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The First Affiliated Hospital of Anhui Medical University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The First Affiliated Hospital of Anhui Medical University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingliang","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The First Affiliated Hospital of Anhui Medical University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyuan","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The First Affiliated Hospital of Anhui Medical University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunlong","family":"Liu","sequence":"additional","affiliation":[{"name":"First Clinical Medical College Anhui Medical University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liao","family":"Guan","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The First Affiliated Hospital of Anhui Medical University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5079-8730","authenticated-orcid":false,"given":"Lei","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The First Affiliated Hospital of Anhui Medical University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongwei","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The First Affiliated Hospital of Anhui Medical University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,14]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)69351-2"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/epi.12550"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)32596-0"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02577-y"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(21)00300-8"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2007.10.007"},{"key":"e_1_2_11_8_1","first-page":"373","article-title":"The universal nature, unequal distribution and antioxidant functions of melatonin and its derivatives","volume":"13","author":"J. 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