{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T14:13:16Z","timestamp":1785247996146,"version":"3.55.0"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,6,14]],"date-time":"2023-06-14T00:00:00Z","timestamp":1686700800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970864"],"award-info":[{"award-number":["81970864"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Chronic rhinosinusitis with nasal polyps (CRSwNP) was potentially due to the epithelial barrier injury. YES\u2010associated protein (YAP) is a multifunctional transcriptional factor and plays versatile roles in the regulation and maintenance of epithelial barrier in different organs and tissues. The purpose of this study is to elucidate possible effect and mechanism of YAP on the epithelial barrier of CRSwNP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Patients were divided into CRSwNP group (<jats:italic>n<\/jats:italic>\u2009=\u200912) and control group (<jats:italic>n<\/jats:italic>\u2009=\u20099). The location of YAP, PDZ\u2010binding transcriptional co\u2010activator (TAZ), and Smad7 were estimated by immunohistochemistry and immunofluorescence. Meanwhile, the expression of YAP, TAZ, Zona occludens\u20101 (ZO\u20101), E\u2010cadherin, and transforming growth factor\u2010beta1 (TGF\u2010\u03b21) were detected by Western blot. After primary human nasal epithelial cells were treated with YAP inhibitor, the expression level of YAP, TAZ, ZO\u20101, E\u2010cadherin, TGF\u2010\u03b21, and Smad7 were measured by Western blot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with the control group, the protein levels of YAP, TAZ, and Smad7 were significantly upregulated, while TGF\u2010\u03b21, ZO\u20101, and E\u2010cadherin were downregulated in CRSwNP. YAP and Smad7 demonstrated lower levels, while the expression of ZO\u20101, E\u2010cadherin, and TGF\u2010\u03b21 rose slightly after YAP inhibitor treatment in primary nasal epithelial cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Higher level of YAP may lead to CRSwNP epithelial barrier injury via the TGF\u2010\u03b21 signaling pathway, and the inhibition of YAP can partially reverse epithelial barrier function.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.907","type":"journal-article","created":{"date-parts":[[2023,6,14]],"date-time":"2023-06-14T13:18:45Z","timestamp":1686748725000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["YES\u2010associated protein\u2010regulated Smad7 worsen epithelial barrier injury of chronic sinusitis with nasal polyps"],"prefix":"10.1002","volume":"11","author":[{"given":"Xiaocong","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology The First Affiliated Hospital of Chongqing Medical University Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0792-0761","authenticated-orcid":false,"given":"Longlan","family":"Shu","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology The First Affiliated Hospital of Chongqing Medical University Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0125-6334","authenticated-orcid":false,"given":"Yijun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology The First Affiliated Hospital of Chongqing Medical University Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4714-0839","authenticated-orcid":false,"given":"Yang","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology The First Affiliated Hospital of Chongqing Medical University Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2459-9612","authenticated-orcid":false,"given":"Xia","family":"Ke","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology The First Affiliated Hospital of Chongqing Medical University Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3675-1845","authenticated-orcid":false,"given":"Jie","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology The First Affiliated Hospital of Chongqing Medical 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