{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T18:10:37Z","timestamp":1774635037729,"version":"3.50.1"},"reference-count":14,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,6,26]],"date-time":"2023-06-26T00:00:00Z","timestamp":1687737600000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>In this study, we investigated the impact of Zibai ointment on wound healing by analyzing the expression levels of two key apoptosis\u2010related factors\u2014B\u2010cell lymphoma 2 (Bcl\u20102) and Bcl\u20102\u2010associated X protein (Bax), in patients following surgery for anal fistula.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We included 90 patients with anal fistulas who were treated in the People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine. Patients were randomly assigned to receive treatment with Zibai ointment (<jats:italic>n<\/jats:italic>\u2009=\u200945) or petroleum jelly (<jats:italic>n<\/jats:italic>\u2009=\u200945). The levels of apoptosis\u2010related factors Bcl\u20102 and Bax were evaluated using enzyme\u2010linked immunosorbent assay (ELISA), while cell apoptosis was assessed using Terminal deoxynucleotidyl transferase (TdT) dUTP Nick\u2010End Labeling (TUNEL) assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results of ELISA showed that on Day 21 after the surgery, the levels of Bcl\u20102 and Bax in the Zibai ointment group were significantly different compared to the petroleum jelly group, with values of (60.11\u2009\u00b1\u20091.31) ng\/mL and (7.05\u2009\u00b1\u20090.01) versus (83.79\u2009\u00b1\u20091.74) ng\/mL and (6.00\u2009\u00b1\u20090.05) ng\/mL, respectively (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Furthermore, light microscopy revealed a large number of apoptotic cells within the field of vision 14 days postsurgery in the Zibai ointment group, and the healing time in the Zibai ointment group was significantly different from that in the petroleum jelly group (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We found that Zibai ointment effectively promoted wound healing in patients following anal fistula surgery, possibly by regulating Bcl\u20102 and Bax apoptosis\u2010related factors.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.912","type":"journal-article","created":{"date-parts":[[2023,6,26]],"date-time":"2023-06-26T12:33:58Z","timestamp":1687782838000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Investigating the role of Zibai ointment on apoptosis\u2010related factors Bcl\u20102 and Bax in wound healing after anal fistula surgery"],"prefix":"10.1002","volume":"11","author":[{"given":"Er\u2010Wei","family":"Cai","sequence":"first","affiliation":[{"name":"The Second Department of Anorectal The Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9411-2972","authenticated-orcid":false,"given":"Cheng","family":"Zhao","sequence":"additional","affiliation":[{"name":"The Second Department of Anorectal The Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Juan","family":"Wang","sequence":"additional","affiliation":[{"name":"Paramedics The Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen\u2010Peng","family":"Xu","sequence":"additional","affiliation":[{"name":"The Second Department of Anorectal The Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Lin","sequence":"additional","affiliation":[{"name":"The Second Department of Anorectal The Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,6,26]]},"reference":[{"issue":"3","key":"e_1_2_9_2_1","first-page":"188","article-title":"Clinical guidelines for the diagnosis and treatment of anal fistula (2006)","volume":"1","author":"Anorectal Branch of China Association of Traditional Chinese Medicine; Colorectal Anal Surgery Group, Surgery Society of Chinese Medical Association; Chinese Association of Integrated Traditional and Western Medicine large intestine anal disease professional committee","year":"2006","journal-title":"Color Med Integ Traditi Chinese Western Med"},{"key":"e_1_2_9_3_1","first-page":"1","volume-title":"Guidelines for the Diagnosis and Treatment of Common Diseases in Anorectal Medicine of Traditional Chinese Medicine","author":"China Association of Traditional Chinese Medicine","year":"2012"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.23162"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1308\/rcsann.2017.0187"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.patbio.2014.02.007"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2013.12.006"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2022.120625"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.14348\/molcells.2017.2299"},{"issue":"8","key":"e_1_2_9_10_1","first-page":"1847","article-title":"Effects of BushenJianpi kaixinfang on cortical inflammation and apoptosis\u2010related proteins Bcl\u20102 and bax induced by d\u2010gal in subacute aging rats","volume":"33","author":"Jin TT","year":"2022","journal-title":"Lishizhen Med Mater Med Res"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2021.116182"},{"issue":"6","key":"e_1_2_9_12_1","first-page":"1450","article-title":"Effects of yangwei jiedu mixture on intestinal tissue of rats with sepsis apoptosis factors Bax,Bcl2 and Caspase3","volume":"36","author":"JIANG Y","year":"2018","journal-title":"Chinese Arch Tradit Chinese Med"},{"issue":"11","key":"e_1_2_9_13_1","first-page":"2979","article-title":"Clinical research progress of TCM promoting wound healing after hemorrhoid fistula","volume":"42","author":"Li CG","year":"2020","journal-title":"Chinese Tradit Patent Med"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.4103\/wjtcm.wjtcm_50_21"},{"issue":"9","key":"e_1_2_9_15_1","first-page":"4108","article-title":"AN a\u2010yue's clinical experience of slow wound healing after operation for patients with anal fistula","volume":"34","author":"Kun L","year":"2019","journal-title":"China J Traditi Chinese Med Pharm"}],"container-title":["Immunity, Inflammation and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iid3.912","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T12:07:23Z","timestamp":1748002043000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/iid3.912"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6]]},"references-count":14,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["10.1002\/iid3.912"],"URL":"https:\/\/doi.org\/10.1002\/iid3.912","archive":["Portico"],"relation":{},"ISSN":["2050-4527","2050-4527"],"issn-type":[{"value":"2050-4527","type":"print"},{"value":"2050-4527","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6]]},"assertion":[{"value":"2023-02-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e912"}}