{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T00:45:35Z","timestamp":1787964335842,"version":"build-2784847793"},"reference-count":60,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,7,12]],"date-time":"2023-07-12T00:00:00Z","timestamp":1689120000000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Impaired apoptosis and proliferation resulted in autoreactive lymphocyte development and inflammation in Rheumatoid arthritis (RA). <jats:italic>TP53<\/jats:italic>, <jats:italic>BAX<\/jats:italic>, <jats:italic>FOXO1<\/jats:italic>, and <jats:italic>RB1<\/jats:italic> are related genes in cell survival, proliferation, and inflammation which could be important in RA development and disease severity. Here we investigated their expression in peripheral blood mononuclear cells (PBMCs) from RA patients in comparison to healthy controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Fifty healthy controls and 50 RA patients were selected. The quantitative real\u2010time polymerase chain reaction was used to assess the gene expression level in PBMCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mRNA expression of <jats:italic>TP53<\/jats:italic> (FC\u2009=\u20090.65, <jats:italic>p<\/jats:italic>\u2009=\u2009.000), <jats:italic>BAX<\/jats:italic> (FC\u2009=\u20090.76, <jats:italic>p<\/jats:italic>\u2009=\u2009.008), <jats:italic>FOXO1<\/jats:italic> (FC\u2009=\u20090.59, <jats:italic>p<\/jats:italic>\u2009=\u2009.000) and <jats:italic>RB1<\/jats:italic> (FC\u2009=\u20090.50, <jats:italic>p<\/jats:italic>\u2009=\u2009.000) were significantly reduced in RA PBMCs. <jats:italic>TP53<\/jats:italic> expression was negatively correlated with miR\u201016\u20105p (<jats:italic>p<\/jats:italic>\u2009=\u2009.032) and <jats:italic>FOXO1<\/jats:italic> expression was negatively correlated with miR\u2010335\u20105p (<jats:italic>p<\/jats:italic>\u2009=\u2009.005) and miR\u201034a\u20105p (<jats:italic>p<\/jats:italic>\u2009=\u2009.014). A positive correlation was seen between <jats:italic>TP53<\/jats:italic> expression and its downstream gene, <jats:italic>BAX<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009=\u2009.001). <jats:italic>FOXO1<\/jats:italic> expression was also negatively correlated with disease activity, DAS28 (<jats:italic>p<\/jats:italic>\u2009=\u2009.021).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>All selected genes have downregulated expression in RA PBMCs which could be correlated with RA pathogenesis by regulating apoptosis, cell survival, inflammatory mediator production, and proliferation. Due to the correlation of miR\u201016\u20105p, miR\u201034a\u20105p, and miR\u2010335\u20105p with <jats:italic>TP53<\/jats:italic> and <jats:italic>FOXO1<\/jats:italic> expression in RA PBMCs, they could be used as future therapeutic targets.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.914","type":"journal-article","created":{"date-parts":[[2023,7,12]],"date-time":"2023-07-12T04:29:30Z","timestamp":1689136170000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Altered expression of apoptosis\u2010related genes in rheumatoid arthritis peripheral blood mononuclear cell and related miRNA regulation"],"prefix":"10.1002","volume":"11","author":[{"given":"Hamidreza","family":"Ebrahimian","sequence":"first","affiliation":[{"name":"Human Genetic Research Center Baqiyatallah University of Medical Sciences Tehran 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