{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T04:15:01Z","timestamp":1768796101495,"version":"3.49.0"},"reference-count":38,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T00:00:00Z","timestamp":1693180800000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Sepsis is a significant contributor to organ function damage or failure that results in intestinal dysfunction. Emodin (Emo) has received much attention for its notable anti\u2010inflammatory and antibacterial properties. We aimed to explore the function of Emo on sepsis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sprague Dawley (SD) rats were pretreated with 20 or 40\u2009mg\/kg of Emo, followed by using cecal ligation and perforation to establish sepsis models. Hereafter, blood glucose levels, biochemical parameters, and inflammatory cytokines were measured. Additionally, ileal myeloperoxidase (MPO)\u00a0activity was also measured. Diamine oxidase (DAO)\u00a0level in plasma, fluorescein isothiocyanate\u2010dextran 40 (FD\u201040)\u00a0level in serum, bacteria number in blood and peritoneal fluid, histopathological changes of ileum, and tight junction (TJ)\u00a0protein expressions in ileum were tested to evaluate the barrier function. Furthermore, CD4+ and CD8+ T cells' percentages were evaluated by flow cytometry. Finally, rats' survival rate was calculated as live rats divided by the total number of rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Emo pretreatment not only decreased blood glucose level, but also downregulated triglyceride (TG),\u00a0alanine aminotransferase (ALT),\u00a0aspartate aminotransferase (AST),\u00a0serum creatinine (SCr),\u00a0blood urea nitrogen (BUN)\u00a0contents for sepsis rats, especially for the high dose of Emo (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Furthermore, Emo inhibited MPO activity and inflammatory factor release (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Crucially, after Emo administration, the barrier function of ileum was enhanced, evidenced by the reduced DAO, FD\u201040\u00a0levels, decreased bacteria number, alleviated pathological damage in ileum and increased TJ protein expressions (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Rats treated with Emo exhibited increased percentages of CD8+ and CD4+\u00a0T cells (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), as well as an improved survival rate.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Emo exhibited a remarkable ability to attenuate sepsis by restoring intestinal dysfunction and improving survival rates, and the mechanism was closely related to anti\u2010inflammatory properties, which provided new solid evidence for the use of Emo in treating sepsis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.942","type":"journal-article","created":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T12:02:37Z","timestamp":1693224157000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Emodin protects against intestinal dysfunction and enhances survival in rat model of septic peritonitis through anti\u2010inflammatory actions"],"prefix":"10.1002","volume":"11","author":[{"given":"Zhongjie","family":"Hua","sequence":"first","affiliation":[{"name":"Department of Emergency Medicine The First People's Hospital of Pinghu Pinghu Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaqin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine The First People's Hospital of Pinghu Pinghu Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiping","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine The First People's Hospital of Pinghu Pinghu Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine The First People's Hospital of Pinghu Pinghu Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2710-6417","authenticated-orcid":false,"given":"Jiali","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine The First People's Hospital of Pinghu Pinghu Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,8,28]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.0287"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2016.10.031"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-019-00987-2"},{"key":"e_1_2_10_5_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2020\/1914031","article-title":"Effect of electroacupuncture at Zusanli (ST36) on sepsis induced by cecal ligation puncture and its relevance to spleen","volume":"2020","author":"Xie D","year":"2020","journal-title":"Evid Based Complementary Altern Med"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCC.0000000000000386"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.01.038"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X20500640"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.0289"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.564674"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3736\/jcim20091215"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2020.106626"},{"key":"e_1_2_10_13_1","first-page":"536","article-title":"Emodin combined with nanosilver inhibited sepsis by anti\u2010inflammatory protection","volume":"7","author":"Li H","year":"2016","journal-title":"Front Pharmacol"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/micc.12061"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2019.02.055"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13020-021-00562-8"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.45777"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.00483"},{"issue":"4","key":"e_1_2_10_19_1","first-page":"1344","article-title":"Effect of astragalosides on long non\u2010coding RNA expression profiles in rats with adjuvant\u2010induced arthritis","volume":"44","author":"Jiang H","year":"2019","journal-title":"Int J Mol Med"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-04231-5"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.15324"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.2147\/DMSO.S273952"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10040843"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11011-022-01017-1"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12010145"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e31827c09f8"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1089\/sur.2017.278"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.919054"},{"issue":"6","key":"e_1_2_10_29_1","first-page":"3521","article-title":"Protective effect of emodin on intestinal epithelial tight junction barrier integrity in rats with sepsis induced by cecal ligation and puncture","volume":"19","author":"Li Y","year":"2020","journal-title":"Exp Ther Med"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.108609"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms16022559"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2017.7259"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPA.0000000000001894"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1701618"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27227883"},{"issue":"6","key":"e_1_2_10_36_1","first-page":"904","article-title":"Beneficial effects of ulinastatin on gut barrier function in sepsis","volume":"138","author":"Jiang L","year":"2013","journal-title":"Indian J Med Res"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2014.09.003"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0145988"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-019-01077-z"}],"container-title":["Immunity, Inflammation and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iid3.942","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T11:21:41Z","timestamp":1698664901000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/iid3.942"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8]]},"references-count":38,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2023,8]]}},"alternative-id":["10.1002\/iid3.942"],"URL":"https:\/\/doi.org\/10.1002\/iid3.942","archive":["Portico"],"relation":{},"ISSN":["2050-4527","2050-4527"],"issn-type":[{"value":"2050-4527","type":"print"},{"value":"2050-4527","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8]]},"assertion":[{"value":"2023-04-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-07-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e942"}}