{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T13:48:19Z","timestamp":1780926499899,"version":"3.54.1"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T00:00:00Z","timestamp":1691452800000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Myocardial injury is the main manifestation of cardiovascular diseases, and previous studies have shown that propofol (PPF) regulates myocardial injury. However, the mechanism of PPF in regulating myocardial injury remains to be further explored. This work aims to analyze the effects of PPF on human cardiomyocyte injury and the underlying mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The regulatory and functional role of PPF and circAPBB2 in human cardiomyocyte injury were analyzed using an in vitro hypoxia\/reoxygenation (H\/R) cell model, which was established by treating human cardiomyocytes (AC16 cells) with H\/R. The study evaluated AC16 cell injury by analyzing cytotoxicity, oxidative stress, inflammation and apoptosis of H\/R\u2010induced AC16 cells. Quantitative real\u2010time polymerase chain reaction was performed to detect circAPBB2, miR\u201018a\u20105p and dual specificity phosphatase 14 (DUSP14) expression. Protein expression was analyzed by Western blot analysis assay. Dual\u2010luciferase reporter assay, RNA pull\u2010down assay and RNA immunoprecipitation assay were performed to identify the associations among circAPBB2, miR\u201018a\u20105p and DUSP14. Cytotoxicity was investigated by cell counting kit\u20108 assay and lactate dehydrogenase activity detection kit. Oxidative stress was evaluated by cellular reactive oxygen species assay kit and superoxide dismutase activity assay kit. The production of tumor necrosis factor\u2010\u03b1 and interleukin\u20101\u03b2 was evaluated by enzyme\u2010linked immunosorbent assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The expression of circAPBB2 and DUSP14 was significantly decreased, while miR\u201018a\u20105p was increased in H\/R\u2010induced AC16 cells when compared with controls. H\/R treatment\u2010induced cytotoxicity, oxidative stress, inflammation and cell apoptosis were attenuated after circAPBB2 overexpression or PPF treatment, whereas these effects were restored by increasing miR\u201018a\u20105p expression. PPF treatment improved the inhibitory effect of ectopic circAPBB2 expression on H\/R\u2010induced cell injury. MiR\u201018a\u20105p silencing ameliorated H\/R\u2010induced AC16 damage by interacting with DUSP14. Mechanically, circAPBB2 acted as a miR\u201018a\u20105p sponge, and miR\u201018a\u20105p targeted DUSP14 in AC16 cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>PPF synergized with circAPBB2 to protect AC16 cells against H\/R\u2010induced oxidative stress, inflammation and apoptosis through the miR\u201018a\u20105p\/DUSP14 pathway.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.952","type":"journal-article","created":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T13:13:13Z","timestamp":1691500393000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Propofol synergizes with circAPBB2 to protect against hypoxia\/reoxygenation\u2010induced oxidative stress, inflammation, and apoptosis of human cardiomyocytes"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4715-0702","authenticated-orcid":false,"given":"Chenghao","family":"Jin","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Beijing Tongren Hospital, Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunnv","family":"Yuan","sequence":"additional","affiliation":[{"name":"Laboratory Medicine The Affiliated Hospital of Yanbian University Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longyi","family":"Piao","sequence":"additional","affiliation":[{"name":"Department of Oncology Jilin Central Hospital of Jilin University Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingcheng","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Oncology Dandong Central Hospital Dandong Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Beijing Tongren Hospital, Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26848"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000518879"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2021.108296"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27384"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-020-01245-4"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-021-00706-7"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-018-0206-4"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/2097954"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.9129"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2016.03.015"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13239-019-00452-z"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2009.01.007"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-7254.2007.00566.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.28897"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.841410"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/1440-1681.13523"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-2273-y"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2020.01.020"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.121.001225"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/ehf2.13814"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s10020-022-00452-1"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s10020-021-00385-1"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcdd5030037"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcdd10030123"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2016.253"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.1c00456"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/iep.12137"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11060983"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s10020-021-00328-w"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.119.256982"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2022.863238"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.00494"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S233672"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1177\/10760296211050642"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12012-021-09670-1"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2021.105941"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201800244RR"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-016-0536-7"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1300989"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1080\/10799893.2020.1831534"}],"container-title":["Immunity, Inflammation and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iid3.952","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T11:19:45Z","timestamp":1698664785000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/iid3.952"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8]]},"references-count":40,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2023,8]]}},"alternative-id":["10.1002\/iid3.952"],"URL":"https:\/\/doi.org\/10.1002\/iid3.952","archive":["Portico"],"relation":{},"ISSN":["2050-4527","2050-4527"],"issn-type":[{"value":"2050-4527","type":"print"},{"value":"2050-4527","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8]]},"assertion":[{"value":"2023-04-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-07-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e952"}}