{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T13:56:39Z","timestamp":1778766999150,"version":"3.51.4"},"reference-count":27,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T00:00:00Z","timestamp":1691452800000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Polysulfides are reported to be involved in various important biological processes. N\u2010acetyl\u2010<jats:sc>l<\/jats:sc>\u2010cysteine polysulfide with 2 sulfane sulfur atoms (NAC\u2010S2) regulates diverse toll\u2010like receptor (TLR) signaling pathways. Here, we aimed to determine the role of NAC\u2010S2 in periodontitis and explore the potential mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A periodontitis mouse model was established by ligating the subgingival between the first and second molars in wild\u2010type, TLR4<jats:sup>\u2010\/\u2010<\/jats:sup>, and Myd88<jats:sup>\u2010\/\u2010<\/jats:sup> mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>NAC\u2010S2 did not affect the proportion of macrophages (CD11b<jats:sup>+<\/jats:sup>F4\/80<jats:sup>+<\/jats:sup>) or neutrophils (CD11b<jats:sup>+<\/jats:sup>GR\u20101<jats:sup>+<\/jats:sup>) in the bone marrow. Mechanically, lipopolysaccharides (LPS), Zymosan A, or poly I: C induced tumor necrosis factor (TNF), interleukin (IL)\u20106, and IL\u20101\u03b2 expression in bone marrow\u2010derived macrophages (BMDMs) could be inhibited by NAC\u2010S2. On the other hand, NAC\u2010S2 suppressed the phosphorylation levels of I\u03baB\u2010\u03b1, p65, and I\u03baB kinase (IKK)\u2010\u03b2 induced by LPS in BMDMs, while LPS induced phosphorylation of ERK1\/2, p38, and transforming growth factor \u03b2\u2010activated kinase 1 (TAK1) could not be affected by NAC\u2010S2. In wild\u2010type periodontitis mice, NAC\u2010S2 administration decreased the cemento\u2010enamel\u2010junction\u2013alveolar bone crest (CEJ\u2010ABC) distance and the relative mRNA expression of TNF, IL\u20106, and IL\u20101\u03b2, while such phenomena could not be observed in TLR4 deficiency or Myd88 deficiency mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>All of these results indicate that NAC\u2010S2 ameliorates TLR4\/NF\u2010\u03baB pathway mediated inflammation in mouse periodontitis model.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.959","type":"journal-article","created":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T13:13:08Z","timestamp":1691500388000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effects of N\u2010acetyl\u2010L\u2010cysteine polysulfides on periodontitis in a mouse model"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7180-280X","authenticated-orcid":false,"given":"Xinxin","family":"Sun","sequence":"first","affiliation":[{"name":"Dental Department, Hejian Hospital of Traditional Chinese Medicine Cangzhou Central Hospital Medical Group Cangzhou Hebei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaru","family":"Sun","sequence":"additional","affiliation":[{"name":"Dental Clinics Cangzhou Central Hospital Cangzhou Hebei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sumin","family":"Cao","sequence":"additional","affiliation":[{"name":"Dental Clinics Cangzhou Central Hospital Cangzhou Hebei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueli","family":"Liu","sequence":"additional","affiliation":[{"name":"Dental Clinics Cangzhou Central Hospital Cangzhou Hebei China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,8,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24119763"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-022-01133-1"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.38"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.4155\/tde-2023-0001"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2023.5263"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.17-0721"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12002"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.763334"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.2147\/CCIDE.S7712"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.5005\/jp-journals-10024-3455"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12903-023-03121-9"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10060967"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb-2020-0074"},{"key":"e_1_2_10_15_1","first-page":"4316","article-title":"Rationale and evidence for the adjunctive use of N\u2010acetylcysteine in multidrug\u2010resistant infections","volume":"27","author":"Oliva A","year":"2023","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2019.02.003"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/00325481.2018.1396876"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3785"},{"key":"e_1_2_10_19_1","doi-asserted-by":"crossref","unstructured":"MainasG SantamariaP IdeM et al.Could dietary restrictions affect periodontal disease? 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