{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T03:27:05Z","timestamp":1772594825982,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T00:00:00Z","timestamp":1691452800000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine the pathogenesis and molecular targets of anaphylaxis caused by hydatid cyst fluid leakage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>First, Balb\/c mice were infected with <jats:italic>Echinococcus granulosus<\/jats:italic>, and then the anaphylaxis model was developed. The mice were separated into: anaphylaxis caused by the cystic echinococcosis group (ANPC), the cystic echinococcosis without anaphylaxis group (CE group), and the normal control group (CTRL). Following this, the spleen tissue was collected for microRNA (miRNA) sequencing. Using bioinformatics analysis, differentially expressed miRNAs\u00a0(DEMs) were identified. Then, through the use of protein\u2013protein interaction (PPI) networks, the key target genes for miRNA regulation associated with echinococcosis\u2010induced anaphylaxis were identified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>ANPC and CE groups have 29 and 39 DEMs\u00a0compared to the CTRL group, respectively. Based on these 25 DEMs, interactions between miRNA and mRNA were screened, and 174 potential target genes were identified. We performed gene ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes pathway\u00a0enrichment analysis on these 174 target genes, and the results revealed that the three pathways with the highest enrichment were the PI3K\u2010Akt signaling pathway, FoxO signaling pathway, and Focal adhesion. The interaction analysis of PPI and miRNA\u2010hub gene networks revealed that interleukin 6 (IL\u20106) was regulated by miR\u2010146a\u20105p and miR\u2010149\u20105p, while IL\u201010 was regulated by miR\u201029b\u20103p and miR\u201029c\u20103. Using reverse transcription polymerase chain reaction, we found that the miRNAs regulating IL\u20106 and IL\u201010 were significantly upregulated in the ANPC group, and there are three pathways involved in that process: Pathways of PI3K\u2010Akt signaling, FoxO signaling, and Focal adhesion. IL\u20106 and IL\u201010 play an important role in cellular pyroptosis and apoptosis. Therefore, the aforementioned results provide significant reference value for elucidating the mechanism of cellular pyroptosis and apoptosis in echinococcosis\u2010induced anaphylaxis, and for formulating tissue and organ protection strategies for patients with cystic echinococcosis when anaphylaxis is triggered by hydatid cyst rupture.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.961","type":"journal-article","created":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T13:09:49Z","timestamp":1691500189000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The regulatory role of differential microRNA expressions on cellular inflammatory factors IL\u20106 and IL\u201010 in <i>Echinococcus granulosus<\/i>\u2010induced anaphylaxis"],"prefix":"10.1002","volume":"11","author":[{"given":"Chun\u2010sheng","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Anesthesiology The First Affiliated of Xinjiang Medical University Urumqi Xinjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Yu","sequence":"additional","affiliation":[{"name":"Shandong Institute of Parasitic Diseases Shandong First Medical University (Shandong Academy of Medical Sciences) Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xilizhati","family":"Kulaixi","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The First Affiliated of Xinjiang Medical University Urumqi Xinjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing\u2010ru","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The First Affiliated of Xinjiang Medical University Urumqi Xinjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianyidan","family":"Abulajiang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The First Affiliated of 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