{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T22:51:51Z","timestamp":1757544711874},"reference-count":76,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2002,1,29]],"date-time":"2002-01-29T00:00:00Z","timestamp":1012262400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2002,4,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Prognostic parameters for axillary node\u2010negative (ANN) breast cancer are still rare. Our aim was to establish potential markers that predict distant metastasis in ANN breast carcinoma and permit detection of the patients with high metastasis risk. A case control study was designed that comprised 64 ANN patients who developed distant metastasis during a 5\u201310 year follow\u2010up period, 64 ANN patients with recurrence\u2010free survival and 64 node\u2010positive (ANP) comparitors. Immunohistochemistry and\/or <jats:italic>in situ<\/jats:italic> hybridization were used to detect nm23, Cathepsin\u2010D (Cath\u2010D), Epidermal Growth Factor Receptor (EGFR) and Laminin Receptor (LR) in 192 cases. A significantly lower expression of both nm23 mRNA and protein was found in the ANN\u2010group with poor prognosis compared with the ANN\u2010group with good prognosis (<jats:italic>p<\/jats:italic> &lt; 0.01). The protein levels of Cath\u2010D, EGFR and LR were significantly higher in the ANN\u2010group with poor prognosis and in the ANP\u2010group compared with the ANN\u2010group with good prognosis (<jats:italic>p<\/jats:italic> &lt; 0.01 or <jats:italic>p<\/jats:italic> &lt; 0.05), but no differences were found between the poor ANN\u2010group and the ANP\u2010group. Multiple regression analysis showed a close correlation of nm23, Cath\u2010D and EGFR expression with occurrence of distant metastasis of ANN breast carcinoma. All markers except nm23 correlated with conventional histopathologic criteria such as tumor grade, margin and vessel invasion. The results suggest the combined detection of nm23, Cath\u2010D and EGFR as predictive markers of distant metastasis in ANN breast cancer patients. Quantitative analysis together with clinicopathologic factors could contribute to estimate the potential risk of metastasis and select individual therapy regimen. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.10136","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T23:13:41Z","timestamp":1030317221000},"page":"754-760","source":"Crossref","is-referenced-by-count":20,"title":["Potential markers predicting distant metastasis in axillary node\u2010negative breast carcinoma"],"prefix":"10.1002","volume":"98","author":[{"given":"Yun","family":"Niu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xilin","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ajuan","family":"Lv","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,1,29]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1999.17.9.2649"},{"key":"e_1_2_6_3_2","first-page":"2766","article-title":"The relationship between nm23, angiogenesis, and the metastatic proclivity of node\u2010negative breast cancer","volume":"58","author":"Heimann R","year":"1998","journal-title":"Cancer Res"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1827.1998.tb03820.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64757-8"},{"key":"e_1_2_6_6_2","first-page":"470","volume-title":"Diseases of the breast","author":"Clark GM","year":"1996"},{"key":"e_1_2_6_7_2","first-page":"519","article-title":"Axillary node metastases in relation to size and location of breast cancers: analysis of 147 patients","volume":"62","author":"Kambouris AA","year":"1996","journal-title":"Am Surg"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.49.11.920"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0748-7983(98)91381-6"},{"key":"e_1_2_6_10_2","first-page":"228","article-title":"The importance of surgery and accurate axillary staging for survival in breast cancer","volume":"25","author":"Grabau DA","year":"1999","journal-title":"Eur J Surg Oncol"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02388136"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1620\/tjem.184.85"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00662138"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.2820741326"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6690373"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(99)00304-4"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(00)00151-9"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/80.3.200"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199804)184:4<401::AID-PATH1220>3.0.CO;2-U"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711720107"},{"key":"e_1_2_6_21_2","first-page":"2325","article-title":"Transfection of human nm23\u2010H1 into the human MDA\u2010MB\u2010435 breast carcinoma cell line: effects on tumor metastatic potential, colonization and enzymatic activity","volume":"8","author":"Leone A","year":"1993","journal-title":"Oncogene"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910550113"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.50.7.586"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0046-8177(94)90003-5"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1993.11.1.36"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(96)90214-2"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006394401199"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19960220)69:1<23::AID-IJC5>3.0.CO;2-Y"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00666181"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00662397"},{"key":"e_1_2_6_31_2","first-page":"217","article-title":"Relationship between c\u2010erbB\u20102 oncoprotein, epidermal growth factor receptor, and estrogen receptor expression in patients with ductal breast carcinoma: association with tumor phenotypes","volume":"10","author":"Harlozinska A","year":"1996","journal-title":"In Vivo"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19960220)69:1<23::AID-IJC5>3.0.CO;2-Y"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19940115)73:2<344::AID-CNCR2820730218>3.0.CO;2-Y"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910600506"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00668209"},{"key":"e_1_2_6_36_2","first-page":"1","article-title":"Laminin receptors and laminin\u2010binding proteins during tumor invasion and metastasis","volume":"13","author":"Castronovo V","year":"1993","journal-title":"Invasion Metastasis"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/85.5.398"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19970315)79:6<1158::AID-CNCR14>3.0.CO;2-Z"},{"key":"e_1_2_6_39_2","first-page":"3987","article-title":"Nm23 expression in breast ductal carcinomas: a ten year follow\u2010up study in a uniform group of node\u2010negative breast cancer patients","volume":"16","author":"Kapranos N","year":"1996","journal-title":"Anticancer Res"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1080\/00313029400169122"},{"key":"e_1_2_6_41_2","first-page":"1417","article-title":"Multivariate analysis of DNA ploidy, p53, c\u2010erbB\u20102 proteins, EGFR, and steroid hormone receptors for prediction of poor short term prognosis in breast cancer","volume":"17","author":"Eissa S","year":"1997","journal-title":"Anticancer Res"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6690336"},{"key":"e_1_2_6_43_2","first-page":"241","article-title":"Estrogens, cathepsin D and metastasis in cancers of the breast and ovary: invasion or proliferation?","volume":"192","author":"Rochefort H","year":"1998","journal-title":"C R Seances Soc Biol Fil"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19990115)85:2<432::AID-CNCR22>3.0.CO;2-J"},{"key":"e_1_2_6_45_2","first-page":"15","volume-title":"Histological typing of breast tumors","year":"1981"},{"key":"e_1_2_6_46_2","first-page":"131","volume-title":"Health statistics","author":"Yang SQ","year":"1998"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/112.4.459"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/85.9.727"},{"key":"e_1_2_6_49_2","first-page":"245","article-title":"Low nm23 protein expression in infiltrating ductal breast carcinoma correlate with reduced patient survival","volume":"139","author":"Barnes R","year":"1991","journal-title":"Am J Pathol"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/0046-8177(94)90004-3"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/0959-8049(92)90069-E"},{"key":"e_1_2_6_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1996.522"},{"key":"e_1_2_6_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1995.31"},{"key":"e_1_2_6_54_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1997.335"},{"key":"e_1_2_6_55_2","first-page":"66","volume-title":"Non\u2010radioactive in situ hybridization tequnich","author":"Lu JP","year":"1996"},{"key":"e_1_2_6_56_2","first-page":"4257","article-title":"Tissue distribution of major matrix metalloproteinases and their transcripts in human breast carcinomas","volume":"19","author":"Lebeau A","year":"1999","journal-title":"Anticancer Res"},{"key":"e_1_2_6_57_2","first-page":"869","article-title":"Study of the expression of the gene encoding Ki\u201067 antigen in human pancreatic cancer using non\u2010radioactive in situ hybridization and immunohistochemistry","volume":"110","author":"Wu Y","year":"1997","journal-title":"Chin Med J Engl"},{"key":"e_1_2_6_58_2","first-page":"854","article-title":"The expression of CD15 mRNA,CD44V6 mRNA and nm23H1 mRNA in breast cancer and their clinical significance","volume":"80","author":"Gu HP","year":"2000","journal-title":"Natl Med J China"},{"key":"e_1_2_6_59_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01806495"},{"key":"e_1_2_6_60_2","unstructured":"1996 Tianjin Science and Technology Publisher Oncology. Tianjin ZG Xiu F Gao TZ Zhang GW Xiu Design and implement of clinical trial 66 77"},{"key":"e_1_2_6_61_2","first-page":"505","article-title":"Reasoning with uncertainty in pathology: artificial neural networks and logistic regression as tools for prediction of lymph node status in breast cancer patients","volume":"12","author":"Marchevsky AM","year":"1999","journal-title":"Mod Pathol"},{"key":"e_1_2_6_62_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19930201)71:3<769::AID-CNCR2820710320>3.0.CO;2-4"},{"key":"e_1_2_6_63_2","doi-asserted-by":"publisher","DOI":"10.1093\/jjco\/29.2.63"},{"key":"e_1_2_6_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0360-3016(99)00553-2"},{"key":"e_1_2_6_65_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6690373"},{"key":"e_1_2_6_66_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6690336"},{"key":"e_1_2_6_67_2","first-page":"131","article-title":"Tumour epidermal growth factor receptor, erbB\u20102 and cathepsin D in node\u2010negative invasive breast cancer: their impact on the selection of patients for systemic adjuvant therapy","volume":"3","author":"Tonkin KS","year":"1999","journal-title":"Cancer Prev Control"},{"key":"e_1_2_6_68_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00668209"},{"key":"e_1_2_6_69_2","doi-asserted-by":"publisher","DOI":"10.3322\/canjclin.47.3.134"},{"key":"e_1_2_6_70_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00689838"},{"key":"e_1_2_6_71_2","doi-asserted-by":"publisher","DOI":"10.1159\/000227358"},{"key":"e_1_2_6_72_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)92407-K"},{"key":"e_1_2_6_73_2","first-page":"30","article-title":"New development of research on tumor metastasis\u2010suppressor gene nm23","volume":"25","author":"Guo FC","year":"1998","journal-title":"J Oncol Med"},{"key":"e_1_2_6_74_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19980425)84:2<109::AID-CNCR7>3.0.CO;2-K"},{"key":"e_1_2_6_75_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/84.16.1266"},{"key":"e_1_2_6_76_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01836956"},{"key":"e_1_2_6_77_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006002823626"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.10136","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.10136","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T05:14:54Z","timestamp":1696914894000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.10136"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,1,29]]},"references-count":76,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2002,4,10]]}},"alternative-id":["10.1002\/ijc.10136"],"URL":"https:\/\/doi.org\/10.1002\/ijc.10136","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,1,29]]}}}