{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T08:56:57Z","timestamp":1790067417171,"version":"4.0.1"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2001,4,19]],"date-time":"2001-04-19T00:00:00Z","timestamp":987638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2001,6,20]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In vitro model cell systems are important tools for studying mechanisms of radiation\u2010induced neoplastic transformation of human epithelial cells. In our study, the human thyroid epithelial cell line HTori\u20103 was analyzed cytogenetically following exposure to different doses of \u03b1\u2010 and \u03b3\u2010irradiation and subsequent tumor formation in athymic nude mice. Combining results from G\u2010banding, comparative genomic hybridization, and spectral karyotyping, chromosome abnormalities could be depicted in the parental line HTori\u20103 and in nine different HTori lines established from the developed tumors. A number of chromosomal aberrations were found to be characteristic for simian virus 40 immortalization and\/or radiation\u2010induced transformation of human thyroid epithelial cells. Common chromosomal changes in cell lines originating from different irradiation experiments were loss of 8q23 and 13cen\u2010q21 as well as gain of 1q32\u2010qter and 2q11.2\u2010q14.1. By comparison of chromosomal aberrations in cell lines exhibiting a different tumorigenic behavior, cytogenetic markers important for the tumorigenic process were studied. It appeared that deletions on chromosomes 9q32\u2010q34 and 7q21\u2010q31 as well as an increased copy number of chromosome 20 were important for the tumorigenic phenotype. A comparative breakpoint analysis of the marker chromosomes found and those observed in radiation\u2010induced childhood thyroid tumors from Belarus revealed a coincidence for a number of chromosome bands. Thus, the data support the usefulness of the established cell system as an in vitro model to study important steps during radiation\u2010induced malignant transformation in human thyroid cells. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.1015","type":"journal-article","created":{"date-parts":[[2004,11,14]],"date-time":"2004-11-14T11:09:40Z","timestamp":1100430580000},"page":"166-177","source":"Crossref","is-referenced-by-count":8,"title":["Clonal chromosomal aberrations in simian virus 40\u2010transfected human thyroid cells and in derived tumors developed after in vitro irradiation"],"prefix":"10.1002","volume":"96","author":[{"given":"Horst","family":"Zitzelsberger","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jochen","family":"Bruch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Smida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludwig","family":"Hieber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clare M.","family":"Peddie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter E.","family":"Bryant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew C.","family":"Riches","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingly","family":"Fung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heinz\u2010Ulrich G.","family":"Weier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manfred","family":"Bauchinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,4,19]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"5653","article-title":"Evidence for the multistep nature of in vitro human epithelial cell carcinogenesis","volume":"50","author":"Rhim JS","year":"1990","journal-title":"Cancer Res"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.3.1174"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/15.3.431"},{"key":"e_1_2_6_5_2","first-page":"757","article-title":"Radiation\u2010induced transformation of SV40\u2010immortalized human thyroid epithelial cells by single and fractionated exposure to gamma\u2010irradiation in vitro","volume":"66","author":"Riches AC","year":"1994","journal-title":"Int J Rad Biol"},{"key":"e_1_2_6_6_2","first-page":"5","article-title":"Radiation\u2010induced neoplastic transformation of human prostate epithelial cells","volume":"56","author":"Kuettel MR","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/095530097143013"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1520-6823(1997)5:3<139::AID-ROI11>3.0.CO;2-1"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001090050169"},{"key":"e_1_2_6_10_2","first-page":"141","article-title":"Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping","volume":"59","author":"Macville M","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_6_11_2","first-page":"135","article-title":"Cytogenetic changes in radiation induced tumors of the thyroid","volume":"59","author":"Zitzelsberger H","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0497-406"},{"key":"e_1_2_6_13_2","doi-asserted-by":"crossref","first-page":"2038","DOI":"10.1182\/blood.V93.6.2038.406a32_2038_2042","article-title":"Spectral karyotype analysis of T\u2010cell acute leukemia","volume":"93","author":"Rowley JD","year":"1999","journal-title":"Blood"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1359641"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1989.387"},{"key":"e_1_2_6_16_2","volume-title":"ISCN: An International System for Human Cytogenetic Nomenclature","author":"Mitelman F","year":"1995"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.273.5274.494"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/1361-6374(199606)4:2<65::AID-BIO4>3.0.CO;2-D"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870100403"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2264(199810)23:2<167::AID-GCC10>3.0.CO;2-L"},{"key":"e_1_2_6_21_2","first-page":"3803","article-title":"Mapping of chromosomal imbalances in pancreatic carcinoma by comparative genomic hybridization","volume":"56","author":"Solinas\u2010Toldo S","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(96)00210-5"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240420103"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1200842"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2300822"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.13.5635"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8469989"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.13.10.6036"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.14.4.2291"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.12.5498"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870140206"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.8.3854"},{"key":"e_1_2_6_33_2","first-page":"6090","article-title":"Evidence for multiple pathways to cellular senescence","volume":"54","author":"Sasaki M","year":"1994","journal-title":"Cancer Res"},{"key":"e_1_2_6_34_2","volume-title":"Catalog of Chromosome Aberrations in Cancer","author":"Mitelman F","year":"1998"},{"key":"e_1_2_6_35_2","first-page":"1107","article-title":"DNA copy number amplifications in human neoplasms: Review of comparative genomic hybridization studies","volume":"152","author":"Knuutila S","year":"1998","journal-title":"Am J Pathol"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65166-8"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2264(199705)19:1<43::AID-GCC7>3.0.CO;2-0"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65407-7"},{"key":"e_1_2_6_39_2","first-page":"547","article-title":"Frequent chromosomal DNA unbalance in thyroid oncocytic (H\u00fcrthle cell) neoplasms detected by comparative genomic hybridization","volume":"79","author":"Tallini G","year":"1999","journal-title":"Lab Invest"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.1015","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.1015","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T23:58:38Z","timestamp":1696895918000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.1015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,4,19]]},"references-count":38,"aliases":["10.1002\/ijc.1015.abs"],"journal-issue":{"issue":"3","published-print":{"date-parts":[[2001,6,20]]}},"alternative-id":["10.1002\/ijc.1015"],"URL":"https:\/\/doi.org\/10.1002\/ijc.1015","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,4,19]]}}}