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We recently described a new uPAR\u2010associated protein (uPARAP), an internalization receptor that interacts with the pro\u2010uPA:uPAR complex. In our study, we generated a specific polyclonal peptide antibody against human uPARAP and used it for the localization of uPARAP in different breast lesions. The affinity\u2010purified antibodies specifically recognized uPARAP in Western blotting and gave a strong signal in immunohistochemistry. The immunohistochemic localization pattern was found to be identical to that of uPARAP mRNA as determined in parallel by <jats:italic>in situ<\/jats:italic> hybridization. uPARAP expression was then studied in both benign and malignant breast lesions. Whereas the normal breast tissue was uPARAP\u2010negative, all benign lesions and ductal carcinoma <jats:italic>in situ<\/jats:italic> lesions showed immunoreactivity in fibroblast\u2010like cells and myoepithelial cells associated with the lesion. In invasive carcinoma, uPARAP immunoreactivity was limited to tumor\u2010associated mesenchymal cells. Double immunofluorescence analysis of invasive ductal carcinoma using antibodies against specific cell markers showed that uPARAP was localized in myofibroblasts and macrophages. No malignant cells, no endothelial cells and no vascular smooth muscle cells showed uPARAP immunoreactivity. We conclude that expression of uPARAP is associated with the abnormal breast and that expression appears in myofibroblasts, macrophages and myoepithelium. We suggest that uPARAP is involved in the clearance of the uPA:uPAR complex as well as other possible ligands during benign and malignant tissue remodeling. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.10227","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T23:13:41Z","timestamp":1030317221000},"page":"656-664","source":"Crossref","is-referenced-by-count":54,"title":["Urokinase receptor\u2010associated protein (<scp>U<\/scp>PARAP) is expressed in connection with malignant as well as benign lesions of the human breast and occurs in specific populations of stromal cells"],"prefix":"10.1002","volume":"98","author":[{"given":"Boye Schnack","family":"Nielsen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fritz","family":"Rank","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars H.","family":"Engelholm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arne","family":"Holm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keld","family":"Dan\u00f8","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niels","family":"Behrendt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,1,29]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0268-9499(94)90717-X"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1999.tb01534.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.1.181"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.17.4645"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.20.4481"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2000.18.5.1135"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.138.11.4902"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0396-287"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.5.2471"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.29.17124"},{"issue":"1","key":"e_1_2_6_12_2","first-page":"25","article-title":"Metalloproteinases and tissue damage","volume":"30","author":"Murphy G","year":"1991","journal-title":"Br J Rheumatol"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1999.tb01524.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)37940-7"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(90)90164-4"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.2741\/Lijnen"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-1002608"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.106.1.87"},{"key":"e_1_2_6_19_2","doi-asserted-by":"crossref","first-page":"2185","DOI":"10.1016\/S0021-9258(18)94159-1","article-title":"Plasminogen activation initiated by single\u2010chain urokinase\u2010type plasminogen activator. 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