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We identified an expressed sequence tag (EST), which we called malignant melanoma\u2010associated 1 (MMA\u20101a), showing evident differential expression between the 2 cell lines. The MMA\u20101a gene is localized on chromosome 21q22.2 and its mRNA exists of 4 exons. Homology search displayed a splice variant of MMA\u20101a that lacks exon 3 and that was called MMA\u20101b. Expression profiles of MMA\u20101a and MMA\u20101b are determined by reverse transciptase polymerase chain reaction (RT\u2010PCR) analysis. Among 30 different normal tissue samples, expression of MMA\u20101a and MMA\u20101b was exclusively found in the testis after a first PCR of 30 cycles. Even more sensitive screening achieved by performing multiple semi\u2010nested RT\u2010PCR showed no or very low expression in the other normal tissues tested. During melanocytic tumor progression, MMA\u20101a and\/or MMA\u20101b exhibited an emergence of expression in primary melanoma (20%) and melanoma metastasis samples (30%) after only 1 round of PCR. Expression of MMA\u20101a and\/or MMA\u20101b was also identified in other tumor cell lines and fresh tumor samples of variable origin, <jats:italic>e.g.<\/jats:italic>, lung, liver, bladder and soft tissues (sarcomas). We conclude that MMA\u20101a and MMA\u20101b are new members of the family of cancer\/testis antigens. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.10241","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T23:09:26Z","timestamp":1030316966000},"page":"547-553","source":"Crossref","is-referenced-by-count":29,"title":["Expression profiling of MMA\u20101a and splice variant MMA\u20101b: New cancer\/testis antigens identified in human melanoma"],"prefix":"10.1002","volume":"98","author":[{"given":"Nicole J.W.","family":"de Wit","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich H.","family":"Weidle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dirk J.","family":"Ruiter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Goos N.P.","family":"van 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