{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T03:58:48Z","timestamp":1790395128160,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2002,2,8]],"date-time":"2002-02-08T00:00:00Z","timestamp":1013126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Tiroler Verein zur F\u00f6rderung der Krebsforschung"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2002,4,20]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To assess the frequency and prognostic impact of Ep\u2010CAM and Her\u20102\/neu overexpression in patients with breast cancer and to determine its relationship with other prognostic markers, 205 breast cancer patients with a median follow\u2010up of 10.8 years were enrolled in this retrospective study. Overexpression of Ep\u2010CAM and Her\u20102\/neu in tumor tissue samples was assessed by immunohistochemistry. Tumors presenting a Her\u20102\/neu 2+ staining were additionally analyzed by FISH to exclude false positive results. Ep\u2010CAM and Her\u20102\/neu overexpression was found in 35.6% and 19.5% of the tumor samples, respectively. Both Ep\u2010CAM and Her\u20102\/neu overexpression were predictive for poor disease\u2010free (DFS) and disease\u2010related overall survival (DROS). Concurrent Ep\u2010CAM and Her\u20102\/neu overexpression was present in 13.2% of tumor specimens and had an additive negative impact on DFS and DROS. This minority of patients had a median time to relapse of only 34 months, whereas the median time to relapse was not reached in the patient population without Her\u20102\/neu and Ep\u2010CAM overexpression. By multivariate analysis Ep\u2010CAM overexpression proved to be an indicator of poor prognosis, independent of tumor size, histologic grade, hormone receptor expression and Her\u20102\/neu overexpression. In conclusion, overexpression of Ep\u2010CAM and Her\u20102\/neu complement each other as predictors for poor prognosis in patients with invasive breast cancer. Determination of these tumor markers should help in assigning breast cancer patients to 1 of 3 distinct risk categories. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.10270","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T23:15:07Z","timestamp":1030317307000},"page":"883-888","source":"Crossref","is-referenced-by-count":94,"title":["Prognostic significance of Ep\u2010CAM AND Her\u20102\/neu overexpression in invasive breast cancer"],"prefix":"10.1002","volume":"98","author":[{"given":"Gilbert","family":"Spizzo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Obrist","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Ensinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Theurl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"D\u00fcnser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Ramoni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eberhard","family":"Gunsilius","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00fcnther","family":"Eibl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregor","family":"Mikuz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00fcnther","family":"Gastl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,2,8]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910380109"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.1.27"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.125.2.437"},{"key":"e_1_2_6_5_2","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1242\/jcs.113.3.409","article-title":"Targets of extinction: identification of genes whose expression is repressed as a consequence of somatic fusion between cells representing basal and luminal mammary epithelial phenotypes","volume":"113","author":"MacDougall JR","year":"2000","journal-title":"J Cell Sci"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)03312-2"},{"key":"e_1_2_6_7_2","first-page":"3317","article-title":"Association of the 323\/A3 surface glycoprotein with tumor characteristics and behavior in human breast cancer","volume":"50","author":"Tandon AK","year":"1990","journal-title":"Cancer Res"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/312513a0"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/319230a0"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(87)92736-X"},{"key":"e_1_2_6_11_2","first-page":"827","article-title":"Evaluation of HER\u20102\/neu (erbB\u20102) status in breast cancer: from bench to bedside","volume":"12","author":"Hanna W","year":"1999","journal-title":"Mod Pathol"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910490109"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910490504"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910450112"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3798106"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1992.10.7.1044"},{"key":"e_1_2_6_17_2","first-page":"4332","article-title":"HER\u20102\/neu amplification predicts poor survival in node\u2010positive breast cancer","volume":"50","author":"Borg A","year":"1990","journal-title":"Cancer Res"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711630205"},{"key":"e_1_2_6_19_2","first-page":"3296","article-title":"Prognostic significance of c\u2010erbB\u20102 and estrogen receptor status in human breast cancer","volume":"51","author":"McCann AH","year":"1991","journal-title":"Cancer Res"},{"key":"e_1_2_6_20_2","first-page":"556","article-title":"Correlation between c\u2010erbB\u20102 amplification and risk of recurrent disease in node\u2010negative breast cancer","volume":"51","author":"Paterson MC","year":"1991","journal-title":"Cancer Res"},{"key":"e_1_2_6_21_2","first-page":"4960","article-title":"Her\u20102\/neu expression in node\u2010negative breast cancer: direct tissue quantitation by computerized image analysis and association of overexpression with increased risk of recurrent disease","volume":"53","author":"Press MF","year":"1993","journal-title":"Cancer Res"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1996.14.10.2702"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/90.18.1346"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1996.14.3.737"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1998.16.8.2659"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200103153441101"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2001.19.2.354"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001099900038"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/90.9.691"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1997.01900040048008"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.139.5.1337"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.108.6.2435"},{"key":"e_1_2_6_33_2","first-page":"865","article-title":"Expression of Ep\u2010CAM in cervical squamous epithelia correlates with an increased proliferation and the disappearance of markers for terminal differentiation","volume":"148","author":"Litvinov SV","year":"1996","journal-title":"Am J Pathol"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199906)188:2<201::AID-PATH339>3.0.CO;2-8"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1994.2631"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1998.4263"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1998.16.4.1340"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19940501)73:9<2359::AID-CNCR2820730919>3.0.CO;2-9"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/35021093"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.10270","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.10270","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,8]],"date-time":"2023-10-08T12:47:45Z","timestamp":1696769265000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.10270"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,2,8]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2002,4,20]]}},"alternative-id":["10.1002\/ijc.10270"],"URL":"https:\/\/doi.org\/10.1002\/ijc.10270","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,2,8]]}}}