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Epstein\u2010Barr virus (EBV) is associated with a number of cancers, including Burkitt lymphoma (BL) and nasopharyngeal carcinoma (NPC) and is suspected to contribute to their tumorigenesis. On average, 8% of gastric carcinomas have been shown to carry this virus. To explore whether the presence of EBV in gastric carcinoma contributes to tumor progression in this predominantly invasive carcinoma, we examined a panel of 2 <jats:italic>in vitro<\/jats:italic> EBV\u2010infected human gastric cancer cell line sublines and their mock\u2010infected AGS parental control line. We found EBV infection caused a marked increase in transmigration of a Matrigel barrier (415% and 303%, <jats:italic>p<\/jats:italic> &lt; 0.05, for the 2 infected lines). This correlated with increased motility of these sublines (233% and 140%, <jats:italic>p<\/jats:italic> &lt; 0.05). As this pattern of increased motility leading to a more pronounced enhancement of invasion has been noted in other tumor cells, we explored the roles of autocrine signaling pathways previously implicated in carcinoma motility and invasion. Inhibitors to the epidermal growth factor receptor (EGFR) (PD153035), phospholipase C (PLC) (U73122), extracellular\u2010signal regulated kinase (ERK)\/mitogen\u2010activated protein kinase (MAPK) (PD089035) and PI\u20103 kinase (Wortmannin) were not informative. These data suggest that EBV increases migration of AGS cells by a mechanism independent of these autocrine growth factor\u2010induced pathways. Instead, we found that the EBV\u2010infected cells presented increased focal adhesion kinase (FAK) phosphorylation. These findings suggest a role for integrin\u2010mediated signaling in promoting EBV\u2010associated invasiveness. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.10382","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T23:29:46Z","timestamp":1030318186000},"page":"644-651","source":"Crossref","is-referenced-by-count":34,"title":["EBV\u2010expressing AGS gastric carcinoma cell sublines present increased motility and 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