{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T09:05:03Z","timestamp":1789203903677,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2002,5,13]],"date-time":"2002-05-13T00:00:00Z","timestamp":1021248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2002,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To determine whether variations in DNA repair genes are related to host DNA damage, we investigated the association between polymorphism in the <jats:italic>XPD<\/jats:italic> gene (codon 199, 312, 751) and the <jats:italic>XRCC1<\/jats:italic> gene (codon 194, 399) and the presence of benzo(a)pyrene diolepoxide adducts to lymphocyte DNA (BPDE\u2010DNA) in a group of male patients with incident lung cancer, all current smokers. BPDE\u2010DNA adducts were analyzed by high\u2010resolution gas chromatography\u2010negative ion chemical ionization\u2010mass spectrometry. <jats:italic>XPD<\/jats:italic> and <jats:italic>XRCC1<\/jats:italic> genotypes were identified by PCR\u2010RFLP. <jats:italic>XRCC1<\/jats:italic> and <jats:italic>XPD<\/jats:italic> genotypes did not affect the levels and proportion of detectable BPDE\u2010DNA adducts. The patients were also genotyped for the <jats:italic>GSTM1<\/jats:italic> polymorphism, given its role in the detoxification of BPDE. Individuals with the <jats:italic>GSTM1<\/jats:italic> deletion had significantly higher levels of BPDE\u2010DNA adducts when they were <jats:italic>XPD\u2010Asp312Asp+Lys751Lys<\/jats:italic> than carriers of at least one variant allele. No such association was found with the <jats:italic>XRCC1<\/jats:italic> genotypes. Because of the small study population (<jats:italic>n<\/jats:italic> = 60), further statistical analysis of possible gene\u2010gene and gene\u2010environment would not be informative. This is the first study analysing the specific BPDE\u2010DNA adduct <jats:italic>in vivo<\/jats:italic> with regard to polymorphic repair genes <jats:italic>(XPD, XRCC1)<\/jats:italic> and xenobiotic metabolizing gene <jats:italic>(GSTM1).<\/jats:italic> Our results raise the possibility that the <jats:italic>XPD\u2010Asp312Asp+Lys751Lys<\/jats:italic> genotype may increase BPDE\u2010DNA damage; this effect might be evident in individuals who are especially likely to have accumulated damage, probably because of lower detoxification capacity and high environmental exposure. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.10463","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T12:27:22Z","timestamp":1033907242000},"page":"9-13","source":"Crossref","is-referenced-by-count":52,"title":["Effect of dna repair gene polymorphisms on BPDE\u2010DNA adducts in human 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