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We have investigated host\u2013tumor interactions between breast carcinoma cells and the lymph node stroma, which may control the outcome of lymph node infiltration. In a previous study, we identified integrin\u2010mediated cell adhesion as a correlate of the metastatic potential of human and rat carcinoma cells. Our present objective was to determine whether lymphatic stromal cells can affect cancer cell growth through the elaboration of growth\u2010modulating factors. Two lymphatic stromal cell lines, ST\u2010A4 and ST\u2010B12, were established from normal rat lymph node stromal cell cultures. SFM conditioned by these cells increased the proliferation of human (Hs578T and MCF\u20107) and rat (TMT\u2010081) breast carcinoma cells by up to 7\u2010fold and augmented their ability to form colonies in semisolid agar by up to 41\u2010fold. This effect was specific as normal, diploid human breast epithelial cells (Hs578Bst), a nontumorigenic, immortalized human breast epithelial cell line (MCF\u201010A) and a nonmetastatic rat mammary carcinoma cell line (MT\u2010W9B) had either no or reduced responses. RT\u2010PCR analysis revealed that both lymph node stromal cell lines expressed mRNA transcripts for multiple growth factors, including IGF\u2010I, EGF, HGF and PDGF\u2010\u03b1, and produced detectable levels of IGF\u2010I, EGF and PDGF\u2010\u03b1, as assessed by Western blotting. Antibody\u2010mediated depletion assays identified IGF\u2010I and EGF as the major mitogenic factors in the CM. The identification of these cells raises the possibility that the lymph node microenvironment may contribute actively to the process of cancer cell dissemination. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.10481","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T12:35:39Z","timestamp":1033907739000},"page":"2-8","source":"Crossref","is-referenced-by-count":64,"title":["Peripheral lymph node stromal cells can promote growth and tumorigenicity of breast carcinoma cells through the release of IGF\u2010I and EGF"],"prefix":"10.1002","volume":"100","author":[{"given":"Christina","family":"LeBedis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keguan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Fallavollita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarek","family":"Boutros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pnina","family":"Brodt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,5,29]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(08)61023-4"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01806081"},{"key":"e_1_2_5_4_2","first-page":"179","article-title":"(2000). 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