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In this study, we have attempted to clarify the biologic characteristics of lipid\u2010depleting factor in a mouse cachexia model. Utilizing differentiated 3T3\u2010L1 adipocytes, we established an assay method quantifying the lipid\u2010depleting activity in plasma derived from colon\u201026\u2010inoculated mice and then analyzed the associated molecular mechanism. Injection (s.c.) of a mouse colon adenocarcinoma cell line, colon\u201026 clone 20, induced cachexia, as evidenced by progressive weight loss. Addition of clone 20\u2013derived cachexigenic, but not clone 5\u2013derived noncachexigenic, plasma to the culture medium of differentiated 3T3\u2010L1 adipocytes reduced the TG content in cultured cells. The ability of the introduced plasma to induce TG loss in 3T3\u2010L1 cells paralleled the body weight changes of tumor\u2010inoculated host mice. Clone 20 plasma, but not clone 5 plasma or recombinant IL\u20106, elicited lipolytic activity, which induced glycerol release from 3T3\u2010L1 cells. Addition of clone 20 plasma to cultured 3T3\u2010L1 adipocytes reduced TG synthesis from [<jats:sup>14<\/jats:sup>C]\u2010glucose compared to clone 5 plasma, indicating that the lipid\u2010depleting activity resulting from addition of clone 20 plasma depended not only on induction of lipolysis but also on inhibition of lipogenesis. Addition of clone 20 plasma to cultured 3T3\u2010L1 adipocytes reduced the quantity of mature SREBP\u20101 in the nucleus of 3T3\u2010L1 cells without affecting PPAR\u2010\u03b3 and C\/EBP\u2010\u03b1. Although TNF\u2010\u03b1 induced apoptosis in 3T3\u2010L1 cells, clone 20 plasma did not. These results suggest that the lipid\u2010depleting factor in clone 20 plasma is different from either IL\u20106 or TNF\u2010\u03b1, and that this factor interfered with not only lipolysis but also lipogenesis through SREBP\u20101 of 3T3\u2010L1 adipocytes. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.10578","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T11:56:23Z","timestamp":1033905383000},"page":"37-45","source":"Crossref","is-referenced-by-count":20,"title":["Molecular analysis of lipid\u2010depleting factor in a colon\u201026\u2010inoculated cancer cachexia 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