{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T06:40:22Z","timestamp":1791009622653,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,8,7]],"date-time":"2002-08-07T00:00:00Z","timestamp":1028678400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Thymoma is one of the most common solid tumors in the mediastinum. The recent development of high\u2010density oligonucleotide arrays provides a unique opportunity to generate gene expression profiles of cells from various stages of tumor progression as it occurs in actual neoplastic tissues. We used oligonucleotide arrays to monitor <jats:italic>in vivo<\/jats:italic> gene expression levels in early\u2010 (stage I or II) and late\u2010 (stage IVa) stage thymoma tissues in 36 patients. These <jats:italic>in vivo<\/jats:italic> gene expression profiles were verified by real\u2010time quantitative RT\u2010PCR using LightCycler. Using both methods, 2 candidate genes were identified that were more highly expressed in advanced\u2010stage thymomas. One was a well\u2010known gene<jats:italic>, c\u2010JUN,<\/jats:italic> and another was an unknown gene, <jats:italic>AL050002. AL050002<\/jats:italic> expression, but not <jats:italic>c\u2010JUN<\/jats:italic> expression, was also correlated with the WHO classification (type B3 <jats:italic>vs.<\/jats:italic> type B1, B2 or AB). The combined use of oligonucleotide microarray and real\u2010time RT\u2010PCR analyses provides a powerful new approach to elucidate the <jats:italic>in vivo<\/jats:italic> molecular events correlated with tumor stage of thymoma. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.10624","type":"journal-article","created":{"date-parts":[[2002,10,8]],"date-time":"2002-10-08T23:02:11Z","timestamp":1034118131000},"page":"342-347","source":"Crossref","is-referenced-by-count":28,"title":["Gene expression analysis of human thymoma correlates with tumor stage"],"prefix":"10.1002","volume":"101","author":[{"given":"Hidefumi","family":"Sasaki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuyuki","family":"Ide","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ichiro","family":"Fukai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanobu","family":"Kiriyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yosuke","family":"Yamakawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshitaka","family":"Fujii","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,8,29]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(78)80131-2"},{"key":"e_1_2_6_3_2","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1001\/archinte.1974.00320200184029","article-title":"The spectrum of disease associated with thymoma: coincidence or syndrome?","volume":"134","author":"Souadjian JV","year":"1974","journal-title":"Arch Intern Med"},{"key":"e_1_2_6_4_2","series-title":"Atlas of tumor pathology","first-page":"55","author":"Rosai J","year":"1976"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.283.5398.83"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1296-1675"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1296-457"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.6.6.492"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1297-1359"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.278.5338.680"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5389.699"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/3282"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.3109\/07853890109002069"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.2144\/97221pf02"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19811201)48:11<2485::AID-CNCR2820481123>3.0.CO;2-R"},{"key":"e_1_2_6_16_2","first-page":"1","volume-title":"World Health Organization International histologic classification of tumors","author":"Rosai J","year":"1999"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204443"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00058052"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01753840"},{"key":"e_1_2_6_20_2","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1159\/000474508","article-title":"p53 and c\u2010jun expression in urinary bladder transitional cell carcinoma: correlation with proliferating cell nuclear antigen (PCNA) histological grade and clinical stage","volume":"31","author":"Skopelitou A","year":"1997","journal-title":"Eur Urol"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(96)90140-9"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01608334"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(85)90086-8"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.3109\/01913128609014592"},{"key":"e_1_2_6_25_2","first-page":"464","article-title":"Immunohistochemical evidence of activate thymocyte proliferation in thymoma. 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