{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T22:27:28Z","timestamp":1791066448061,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,9,6]],"date-time":"2002-09-06T00:00:00Z","timestamp":1031270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2002,11,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of our study was to investigate the physical state and the viral load of HPV\u201016 in tonsillar cancer and to correlate these findings with clinical outcome. To distinguish between integrated and episomal forms of HPV, 22 fresh\u2010frozen tonsillar cancer samples were analysed by a method based on restriction enzyme cleavage, ligation and PCR (<jats:italic>rli<\/jats:italic>PCR). HPV\u201016 was detected in 11\/22 and HPV\u201033 in 1\/22 of the cancers, hence 12\/22 (55%) of the tumours were HPV positive. Only extrachromosomal forms of HPV\u201016 were observed. Full\u2010length episomal HPV was detected exclusively in 7\/11 of the cancers, whereas both full\u2010length and deleted forms of episomal HPV\u201016 were found in parallel in 2 other tumours. In 1 tumour only a deleted episomal form of HPV\u201016 was present. In the remaining HPV\u201016 positive tumour both full\u2010length episomal as well as an 11 kbp PCR product were detected and if the 11 kbp product contained integrated HPV, or was off\u2010size linearised episomal could not be determined. In 2 cervical cancer controls, HPV\u201016 was integrated and could be chromosome located. HPV\u201016 was quantified by real\u2010time PCR and most tonsillar cancers contained between 10 to a few hundred copies of HPV per \u03b2\u2010actin. The 6 patients with tumour sections with \u2265190 HPV\u201016 copies\/\u03b2\u2010actin remained tumour free (<jats:italic>p<\/jats:italic> = 0.026) and had a better survival rate (<jats:italic>p<\/jats:italic> = 0.039) when compared to the 5 patients with tumours sections with \u226460 HPV\u201016 copies\/\u03b2\u2010actin. In conclusion, HPV\u201016 is mainly episomal in tonsillar cancer. The viral load showed a wide distribution and the clinical outcome in our study was better when the HPV load was higher. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.10669","type":"journal-article","created":{"date-parts":[[2002,10,17]],"date-time":"2002-10-17T19:55:50Z","timestamp":1034884550000},"page":"152-158","source":"Crossref","is-referenced-by-count":184,"title":["Human papillomavirus type 16 is episomal and a high viral load may be correlated to better prognosis in tonsillar 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