{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T14:40:44Z","timestamp":1697553644775},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2002,10,16]],"date-time":"2002-10-16T00:00:00Z","timestamp":1034726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2002,12,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A panel of monoclonal antibodies specific to Hong Kong Chinese nasopharyngeal carcinoma (NPC)\u2010associated Epstein\u2010Barr virus (EBV) latent membrane protein 1 (LMP1) variants has been generated. These monoclonal antibodies not only differentiate the Hong Kong Chinese NPC\u2010associated LMP1 variants from the prototype B95\u20108 LMP1, derived from Caucasian infectious mononucleosis, but also differentiate the 2 highly homologous LMP1 deletion variants commonly found in Hong Kong primary NPC. The predominant deletion type variant, DV\u2010Asp335, is characterized by an aspartic acid at residue 335 located in the cytoplasmic C\u2010terminal region, whereas the other minor deletion variant, DV\u2010Gly335, has a glycine in the same residue position. 335D is hitherto found predominantly in LMP1 of the China 1 strain in association with NPC in the Chinese populations located in southern China and Malaysia. These antibodies, which are applicable in ELISA, immunofluorescence, immunoprecipitation, immunoblotting and immunohistochemistry on paraffin sections, are the first variant\u2010specific anti\u2010LMP1 monoclonal antibodies produced, and will be useful in investigating the functional significance of 335D in NPC. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.10773","type":"journal-article","created":{"date-parts":[[2002,11,14]],"date-time":"2002-11-14T13:46:36Z","timestamp":1037281596000},"page":"492-498","source":"Crossref","is-referenced-by-count":5,"title":["Generation of monoclonal antibodies against Hong Kong nasopharyngeal carcinoma\u2010associated Epstein\u2010Barr virus latent membrane protein 1 (LMP1)"],"prefix":"10.1002","volume":"102","author":[{"given":"Ben Chung Lap","family":"Chan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ka 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