{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T08:40:09Z","timestamp":1762504809710},"reference-count":51,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,1,13]],"date-time":"2003-01-13T00:00:00Z","timestamp":1042416000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2003,4,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>CD4<jats:sup>+<\/jats:sup> Th cells that are restricted by MHC class II molecules play an important role in the induction of antitumor immune responses. We have established a stable CD4<jats:sup>+<\/jats:sup> Th cell clone (Th35\u20101A) from the PBMCs of a patient with primary cutaneous melanoma. The Th cell clone is noncytolytic and proliferates specifically in the presence of irradiated autologous melanoma cells or autologous EBV\u2010transformed B cells pulsed with melanoma tumor cell lysates. Th35\u20101A produces IFN\u2010\u03b3 (a Th1\u2010type cytokine) after autologous tumor cell stimulation, and its proliferative reactivity is HLA class II\u2013restricted. Th cells showed helper activity for PWM responses of PBMCs. Using a panel of HLA class II\u2013matched and unmatched EBV\u2010B cells as APCs and allogeneic melanoma tumor cell lysate as stimulant, DR7 was delineated as the HLA class II restriction element used by the Th cell clone. In agreement with these results, transfection of an allogeneic melanoma cell line with HLA\u2010DR7 isolated from autologous EBV\u2010B cells rendered the cell line stimulatory for Th35\u20101A cells. Specificity studies using autologous EBV\u2010B cells (EBV\u2010B35) pulsed with a panel of allogeneic tumor cell lysates of various tissue origins indicated that the Th cell clone recognizes an antigen shared by melanoma and glioma cells. The availability of the Th cell clone may lead to the development of new therapies against melanoma, using adoptive Th cell transfer and\/or active immunization with a shared Th cell antigen. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.10964","type":"journal-article","created":{"date-parts":[[2003,2,6]],"date-time":"2003-02-06T01:52:07Z","timestamp":1044496327000},"page":"362-368","source":"Crossref","is-referenced-by-count":10,"title":["A CD4<sup>+<\/sup>, HLA\u2010DR7\u2010restricted T\u2010helper lymphocyte clone recognizes an antigen shared by human malignant melanoma and 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