{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T08:06:17Z","timestamp":1765267577792},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2003,2,13]],"date-time":"2003-02-13T00:00:00Z","timestamp":1045094400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2003,5,20]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Histologic examination lacks the sensitivity to detect micrometastases in gastric cancer lymph nodes. In the present study, we applied a real\u2010time RT\u2010PCR approach to the quantitative detection of micrometastases in gastric cancer lymph nodes and compared diagnostic power with routine histology and immunohistochemistry. We studied 392 lymph nodes from 21 gastric cancer patients who underwent curative surgery. Real\u2010time quantitative RT\u2010PCR was performed on a LightCycler instrument using a hybridization probe for carcinoembryonic antigen (CEA) and cytokeratin\u201020 (CK20) as marker genes. Immunohistochemistry with antibodies to wide\u2010keratin was also performed in the lymph nodes to compare the sensitivity and specificity. Median (average) values of CEA mRNA in lymph nodes in patients with histology<jats:sup>+<\/jats:sup>, immunohistochemistry<jats:sup>+<\/jats:sup>\/histology<jats:sup>\u2212<\/jats:sup>, immunohistochemistry<jats:sup>\u2212<\/jats:sup>\/histology<jats:sup>\u2212<\/jats:sup> and negative control results were 4,600 (16,000), 200 (400), 0 (9.8) and 0 (0.6), respectively. There were some false\u2010negative results with simple CEA and CK20 real\u2010time RT\u2010PCR due to the presence of low gene\u2010expressing gastric cancers as revealed by CEA and CK20 immunohistochemistry. CEA in combination with CK20 (duplex) real\u2010time RT\u2010PCR partially covered this weakness. Consequently, all 71 histology<jats:sup>+<\/jats:sup> lymph nodes were positive for duplex real\u2010time RT\u2010PCR as well as wide\u2010keratin immunohistochemistry. Positivity rates by histology, wide\u2010keratin immunohistochemistry and duplex real\u2010time RT\u2010PCR were 18.0% (71\/392), 20.9% (82\/392) and 25.8% (101\/392), respectively. In 2 of 8 patients with pT1N0, positive lymph nodes were observed by real\u2010time RT\u2010PCR but not by immunohistochemistry. These results indicate that duplex quantitative real\u2010time RT\u2010PCR is the most sensitive method for detecting micrometastases and useful for evaluating the prognostic significance of lymph node micrometastasis in gastric cancer patients. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.11031","type":"journal-article","created":{"date-parts":[[2003,3,28]],"date-time":"2003-03-28T12:49:30Z","timestamp":1048855770000},"page":"136-143","source":"Crossref","is-referenced-by-count":52,"title":["Quantitative detection of micrometastases in the lymph nodes of gastric cancer patients with real\u2010time RT\u2010PCR: A comparative study with 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