{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T22:59:14Z","timestamp":1791241154018,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,3,10]],"date-time":"2003-03-10T00:00:00Z","timestamp":1047254400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008977","name":"University of Ulm","doi-asserted-by":"crossref","award":["P.641\/P.677\/P.744"],"award-info":[{"award-number":["P.641\/P.677\/P.744"]}],"id":[{"id":"10.13039\/501100008977","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Rudolf and Clothilde Eberhardt-Stiftung, Germany","award":["D1699"],"award-info":[{"award-number":["D1699"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2003,6,20]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Using comparative genomic hybridization (CGH), we present a genome\u2010wide screening of a mixed mesenchymal\u2013epithelial hepatoblastoma, its recurrence and 2 novel hepatoblastoma cell lines raised from the ascites, 18 (HepU1) and 23 (HepU2) months after diagnosis of a hepatoblastoma in a 35\u2010month\u2010old boy. Both cell lines were also characterized by GTG\u2010banding, multicolor\u2010fluorescence <jats:italic>in situ<\/jats:italic> hybridization (M\u2010FISH) and multicolor banding (M\u2010Band). On the basis of CGH, we compared the cytogenetics of histologically different tumor areas of the parental tumor and its recurrence with the hepatoblastoma cell lines. We found different CGH profiles in the parental tumor <jats:italic>rev ish enh<\/jats:italic>(1q31\u2013q32,8p,12,17,20,X), <jats:italic>dim<\/jats:italic>(4q34\u2013q35,18q23)[cp] and its recurrence <jats:italic>rev ish enh<\/jats:italic>(8q24,17,Xq26\u2013q28), <jats:italic>dim<\/jats:italic>(7q11.2\u2013q21,13q34)[cp]. Although both epithelial cell lines were obtained at different times and the clonal ancestor of HepU2 had been exposed to a higher cumulative dose of chemotherapy, HepU1 and HepU2 have an identical karyotype: 48\u201056,XY,+Y,dup(2)(q32\u2013q34),t(3;4)(q21;q34),+8,+12,+13, +17,+t(18;19)(q21;q?),+20[cp] and identical CGH profiles: <jats:italic>rev ish enh<\/jats:italic>(2q24\u2013q33,8,12,13q,17,20), <jats:italic>dim<\/jats:italic>(4q34\u2013q35,18q22\u2013q23). In common with previously described hepatoblastoma cell lines, HepU1 and HepU2 demonstrate a gain of chromosome 20. The <jats:italic>in situ<\/jats:italic> aberrations most closely resembling that of HepU1 and HepU2 were found in areas of fetal\u2013embryonal differentiation of the primary tumor. Interestingly, both cell lines mimic this histology in their three\u2010dimensional growth pattern <jats:italic>in vitro<\/jats:italic>. HepU1 and HepU2 are thus cytogenetically and phenotypically highly characteristic of fetal\u2013embryonal hepatoblastoma. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.11082","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T15:07:07Z","timestamp":1050419227000},"page":"347-352","source":"Crossref","is-referenced-by-count":10,"title":["Two novel <i>in vitro<\/i> human hepatoblastoma models, HepU1 and HepU2, are highly characteristic of fetal\u2013embryonal differentiation in hepatoblastoma"],"prefix":"10.1002","volume":"105","author":[{"given":"Stefanie","family":"Scheil","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Hagen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silke","family":"Br\u00fcderlein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivo","family":"Leuschner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Behnisch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"M\u00f6ller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,3,10]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(01)00588-X"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(99)00203-4"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1442-200x.2001.01380.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(99)00033-3"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-911X(200003)34:3<165::AID-MPO1>3.0.CO;2-T"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(01)00622-7"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.3109\/08880019709028777"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(00)00323-X"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1054\/bjoc.2000.1390"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2264(200002)27:2<196::AID-GCC12>3.0.CO;2-D"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2001.tb01172.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1159\/000057009"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1159\/000015371"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64567-1"},{"key":"e_1_2_6_16_2","first-page":"1","article-title":"Establishment 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