{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T23:59:58Z","timestamp":1773791998974,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,8,6]],"date-time":"2003-08-06T00:00:00Z","timestamp":1060128000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2003,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Isolation of single cells permits analysis of DNA or RNA from individual cells among heterogeneous populations. This technique is particularly useful in the study of classical Hodgkin's lymphoma (cHL) due to the scarcity of H\/RS tumor cells among large numbers of reactive leukocytes. In a previous study, we found a high frequency of dual <jats:italic>LMP\u20101<\/jats:italic> variant (concurrent presence of deleted and nondeleted variants) in cHL from whole\u2010tissue sections. For the present study, we applied a single\u2010cell isolation technique to determine the <jats:italic>LMP\u20101<\/jats:italic> oncogene variant in EBV\u2010associated H\/RS cells. Five cases of EBV\u2010infected cHL, containing nondeleted (<jats:italic>n<\/jats:italic> = 1), deleted (<jats:italic>n<\/jats:italic> = 1) and dual infection (<jats:italic>n<\/jats:italic> =3) based on whole\u2010tissue section analysis, were selected for study. Paraffin\u2010embedded tissue sections were stained with antibody to LMP\u20101 and positively stained H\/RS cells isolated using a semiautomated micromanipulator. Each isolated single cell was subjected to PCR for amplification of the <jats:italic>LMP\u20101<\/jats:italic> gene flanking the 30 bp deletion region and Xho1 restriction site. Cases with either nondeleted variant or the deleted variant showed similar <jats:italic>LMP\u20101<\/jats:italic> variant expression in isolated single H\/RS cells. However, 1 of the 3 cases with dual variants showed only the deleted variant in H\/RS cells. The other 2 cases showed mixed patterns of deleted, nondeleted and dual <jats:italic>LMP\u20101<\/jats:italic> variants in isolated single H\/RS cells. All cases showed loss of the Xho1 restriction site, with the exception of the case with nondeleted <jats:italic>LMP\u20101<\/jats:italic>. Results of single\u2010H\/RS cell analysis of the Xho1 restriction site concur with those of whole\u2010tissue section amplification. A mixed pattern of <jats:italic>LMP\u20101<\/jats:italic> variants was observed in isolated H\/RS cells, and it is speculated that this is due to the accumulation of mutation and deletion events. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.11395","type":"journal-article","created":{"date-parts":[[2003,8,27]],"date-time":"2003-08-27T13:41:56Z","timestamp":1061991716000},"page":"250-255","source":"Crossref","is-referenced-by-count":3,"title":["Dual variant of Epstein\u2010Barr virus in Hodgkin\/Reed\u2010Sternberg cells: Single\u2010cell PCR study on <i>latent membrane protein\u20101 gene<\/i>"],"prefix":"10.1002","volume":"107","author":[{"given":"Lian\u2010hua","family":"Kim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suat\u2010cheng","family":"Peh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sibrand","family":"Poppema","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,8,6]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"219","article-title":"Molecular studies on single cells harvested by micromanipulation from archival sections previously stained by immunohistochemistry or nonisotopic in situ hybridisation","volume":"76","author":"d'Amore F","year":"1997","journal-title":"Lab 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