{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T13:30:27Z","timestamp":1770903027858,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,10,27]],"date-time":"2003-10-27T00:00:00Z","timestamp":1067212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Tumorzentrum Mannheim\/Heidelberg"},{"DOI":"10.13039\/100000884","name":"Cancer Research Institute","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000884","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2004,1,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although the existence of a humoral response against tumor\u2010associated antigens is well appreciated, a systematic analysis of its possible induction by the tumor remains missing. We compared the specific IgG response of Stage IV melanoma patients during vaccination. Patients had been treated within 2 clinical trials with autologous tumor cells gene\u2010modified for IL\u20107 or IL\u201012. A panel of 27 tumor\u2010associated antigens (HD\u2010MM\u201001 to HD\u2010MM\u201027) was isolated by a SEREX screening of a testis cDNA library using a pool of 5 sera from patients after vaccination. All antigens were retested with individual sera of 12 patients both pre\u2010 and post\u2010vaccination. A serological response was induced during vaccination against 18 antigens. Remarkably, induction was detected only in patients included in the screening pool. The low overlap between sero\u2010reactivity of the 12 patients suggested a very individualized immunological reaction. Two of 5 sera included in the screening pool exhibited a high frequency of induced humoral responses. The same patients had been shown to have a high Karnovsky index and had generated lytic cytotoxic T cells against the tumor. Besides 2 known cancer\u2010germline genes (SCP\u20101 and PLU\u20101), the other isolated antigens were expressed in a non\u2010tumor\u2010specific fashion as analyzed by virtual Northern blot or RT\u2010PCR. The properties of homologues to several of the identified tumor\u2010antigens, especially PLU\u20101, SCP\u20101, DNEL2, CLOCK, and PIASx\u2010\u03b1, suggest further investigation of their possible function in malignant melanoma. We conclude that a strong humoral response against tumor\u2010associated antigens is inducible by tumor cells and that this response is very individual. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.11537","type":"journal-article","created":{"date-parts":[[2004,6,2]],"date-time":"2004-06-02T14:21:46Z","timestamp":1086186106000},"page":"307-313","source":"Crossref","is-referenced-by-count":20,"title":["Humoral immune response against melanoma antigens induced by vaccination with cytokine gene\u2010modified autologous tumor 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