{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T03:40:57Z","timestamp":1791776457212,"version":"4.3.4"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2003,12,4]],"date-time":"2003-12-04T00:00:00Z","timestamp":1070496000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2004,3,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    DNA polymerase \u03b8 (Pol \u03b8) is a recently identified family A polymerase that contains an intrinsic helicase domain.\n                    <jats:italic>Drosophila<\/jats:italic>\n                    Pol \u03b8 mutants are hypersensitive to bifunctional DNA crosslinking agents and exhibit an elevated frequency of spontaneous chromosomal aberrations, suggesting a role for Pol \u03b8 in repair of DNA interstrand crosslinks and in the general maintenance of genome stability. To investigate a possible involvement of Pol \u03b8 in tumorigenesis, we have examined its expression in various normal and malignant tissues. Paired tumor and adjacent nontumorous tissues from patients with lung (\n                    <jats:italic>n<\/jats:italic>\n                    = 27), stomach (\n                    <jats:italic>n<\/jats:italic>\n                    = 28) and colon (\n                    <jats:italic>n<\/jats:italic>\n                    = 26) cancer, as well as 26 normal human tissues, were examined for Pol \u03b8 expression by RT\u2010PCR, Northern or Western blot analysis. Pol \u03b8 was predominantly expressed in primary lymphoid organs including the fetal liver, thymus and bone marrow where lymphocyte progenitors undergo V(D)J rearrangements of their antigen receptor genes. In addition, Pol \u03b8 expression was upregulated in germinal center B cells, in which class switch recombination of the immunoglobulin genes occurs. Examination of Pol \u03b8 expression in matched cancer specimens revealed that Pol \u03b8 was barely detectable in the nontumorous tissues but was upregulated in 17 of 27 (63%) lung, 11 of 28 (39%) stomach and 20 of 26 (77%) colon cancers. Moreover, patients with high levels of Pol \u03b8 expression had a significantly poorer clinical outcome compared with those expressing low levels of Pol \u03b8. These results implicate that Pol \u03b8 may have a specialized function in lymphocytes and that its overexpression may contribute to tumor progression. \u00a9 2003 Wiley\u2010Liss, Inc.\n                  <\/jats:p>","DOI":"10.1002\/ijc.11666","type":"journal-article","created":{"date-parts":[[2004,1,16]],"date-time":"2004-01-16T05:00:36Z","timestamp":1074229236000},"page":"9-16","source":"Crossref","is-referenced-by-count":141,"title":["DNA polymerase \u03b8 is preferentially expressed in lymphoid tissues and upregulated in human 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