{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T02:17:48Z","timestamp":1744165068647},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,10,5]],"date-time":"2001-10-05T00:00:00Z","timestamp":1002240000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2002,1,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Suppression subtractive hybridization, comparing mRNA expression profiles of common nevocellular nevi and melanoma metastases, was used to identify potential markers of melanoma progression. From the metastases we isolated XAGE\u20101b, a 470 bp transcript of the XAGE\u20101 gene. In general, expression of XAGE\u20101b was much more prominent than expression of the longer XAGE\u20101 transcript, isolated from Ewing's sarcoma. The XAGE\u20101b open\u2010reading frame codes for a putative protein of 81 amino acids, harboring a functional bipartite nuclear localization signal and a C\u2010terminal acidic transcription\u2010activation\u2010like domain. On the nucleotide level, XAGE\u20101b has a 50% homology with members of the GAGE family. However, homology between the corresponding proteins is weak. Expression of XAGE\u20101b in normal tissues was mainly restricted to testis, while placenta and brain were sporadically positive. In human tumor cell lines as well as in human tumor lesions, expression was most frequently found in melanocytic tumors and Ewing's sarcoma. In the different stages of melanocytic tumor progression, expression was exclusively seen in melanoma metastases (38%; <jats:italic>n<\/jats:italic> = 61), while all tested common and atypical nevi (<jats:italic>n<\/jats:italic> = 10) as well as primary melanomas (<jats:italic>n<\/jats:italic> = 8) were negative. Upregulation of expression after treatment with demethylating agent 5\u2010aza\u20102\u2032\u2010deoxycytidine was detected in 1 of 4 human melanoma cell lines tested. The XAGE\u20101 gene consists of 4 exons and is located on chromosome Xp11.21\u2013Xp11.22. After transfection into COS cells, the corresponding protein can direct the coupled fluorescent protein to the nucleus, showing a distinct speckled staining aspect. Our data imply the nuclear cancer\/testis\u2010associated XAGE\u20101b to be a marker for late melanocytic tumor progression. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.1584","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T22:13:08Z","timestamp":1030313588000},"page":"195-204","source":"Crossref","is-referenced-by-count":40,"title":["Characterization of XAGE\u20101b, a short major transcript of cancer\/testis\u2010associated gene XAGE\u20101, induced in melanoma metastasis"],"prefix":"10.1002","volume":"97","author":[{"given":"Albert J.W.","family":"Zendman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annemieke A.","family":"van Kraats","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anneke I.","family":"den 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