{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T12:28:36Z","timestamp":1790166516914,"version":"4.1.0"},"reference-count":55,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2001,10,18]],"date-time":"2001-10-18T00:00:00Z","timestamp":1003363200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Downregulation of major histocompatibility complex (MHC) molecules by tumor cells impairs cellular immune recognition and contributes to inefficient cell\u2010mediated tumor eradication. Low or lack of expression of MHC molecules is frequently observed in early developmental or embryonically derived tumor cells. Considering the central role of the class II transactivator (CIITA) in MHC class II\u2010 and class I\u2010mediated antigen presentation, we compared the induction of CIITA by interferon\u2010\u03b3 (IFN\u2010\u03b3) in a diverse panel of developmental and more differentiated tumor cell lines. In contrast to the more differentiated tumor cell lines, none of the developmental tumor cell lines were capable of expressing CIITA after treatment with IFN\u2010\u03b3. Remarkably, in transient transfection assays, CIITA promoter IV (CIITA\u2010PIV) was found to be induced by IFN\u2010\u03b3. Southern blot analysis of genomic DNA obtained from the developmental tumor cell lines indicated that the absence of endogenous CIITA induction was due to methylation of the CIITA\u2010PIV region. Exposure to 5\u2010azacytidine restored induction of CIITA and congruent HLA\u2010DRA expression in these cells. The observation that only developmental tumor cell lines, originating from various tissues, employ methylation to silence CIITA expression may reflect the natural status of CIITA expression during early development rather than oncogenic transformation. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.1623","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T16:33:59Z","timestamp":1030293239000},"page":"501-507","source":"Crossref","is-referenced-by-count":56,"title":["Lack of ifn\u2010\u03b3\u2010mediated induction of the class II transactivator (CIITA) through promoter methylation is predominantly found in developmental tumor cell lines"],"prefix":"10.1002","volume":"97","author":[{"given":"Nienke","family":"van der Stoep","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Biesta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin","family":"Quinten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.","family":"van den Elsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,12,29]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(05)80090-X"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/1074-7613(94)90039-6"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.2.765"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/7.8.1295"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/1074-7613(95)90033-0"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/1074-7613(95)90034-9"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80348-9"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80636-6"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80347-7"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6165083"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1988.tb00732.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.08.040190.003341"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.10.040192.000305"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-313X.1989.tb00481.x"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.144.10.4068"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(89)90206-2"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19881101)62:9<1988::AID-CNCR2820620919>3.0.CO;2-0"},{"key":"e_1_2_6_19_2","first-page":"510","article-title":"An evaluation of the prognostic significance of HLA\u2010DR expression in axillary\u2010node\u2010negative breast cancer","volume":"116","author":"Sheen\u2010Chen SM","year":"1994","journal-title":"Surgery"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/jso.2930590111"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1986.tb01518.x"},{"key":"e_1_2_6_22_2","doi-asserted-by":"crossref","first-page":"3766","DOI":"10.4049\/jimmunol.138.11.3766","article-title":"Lack of expression of HLA class I and class II molecules on the human oocyte","volume":"138","author":"Dohr GA","year":"1987","journal-title":"J Immunol"},{"key":"e_1_2_6_23_2","doi-asserted-by":"crossref","first-page":"4157","DOI":"10.4049\/jimmunol.140.12.4157","article-title":"Lack of HLA class I and class II antigens on human preimplantation embryos","volume":"140","author":"Desoye G","year":"1988","journal-title":"J Immunol"},{"key":"e_1_2_6_24_2","first-page":"457","article-title":"Class 1 major histocompatibility complex antigens on human extra\u2010villous trophoblast","volume":"52","author":"Redman CWG","year":"1984","journal-title":"Immunology"},{"key":"e_1_2_6_25_2","first-page":"101","article-title":"The role of surface HLA\u2010A,B,C molecules in tumour immunity","volume":"13","author":"M\u00f6ller P","year":"1992","journal-title":"Cancer Surv"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(96)10075-X"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0198-8859(99)00156-1"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002510050007"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0198-8859(00)00159-2"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.12.6330"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.14.9.1156"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.16.6051-6061.2000"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.12.6324"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1997.1121"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.10.2851"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80468-9"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.1.431"},{"key":"e_1_2_6_38_2","doi-asserted-by":"crossref","first-page":"233","DOI":"10.4049\/jimmunol.160.1.233","article-title":"Identification of distinct regions of 5\u2032 flanking DNA that mediate constitutive, IFN\u2010\u03b3, STAT1, and TGF\u2010\u03b2\u2010regulated expression of the class II transactivator gene","volume":"160","author":"Piskurich JF","year":"1998","journal-title":"J Immunol"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.4.1367"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8016643"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830191224"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.10.7.3792"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.7.8.2745"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.15.4293"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.9.7.3136"},{"key":"e_1_2_6_46_2","first-page":"3694","article-title":"Beta 2\u2010microglobulin expression in human embryonal neuroblastoma reflects its developmental regulation","volume":"50","author":"Cooper MJ","year":"1990","journal-title":"Cancer Res"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199901)187:2<191::AID-PATH206>3.0.CO;2-3"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.8.4143"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(02)70795-1"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118958"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.163.8.4224"},{"key":"e_1_2_6_52_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.53.4.286"},{"key":"e_1_2_6_53_2","doi-asserted-by":"publisher","DOI":"10.1006\/scdb.1998.0225"},{"key":"e_1_2_6_54_2","doi-asserted-by":"publisher","DOI":"10.1006\/scbi.1999.0133"},{"key":"e_1_2_6_55_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/21.3.461"},{"key":"e_1_2_6_56_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830190710"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.1623","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.1623","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T17:09:49Z","timestamp":1697562589000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.1623"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,12,29]]},"references-count":55,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,2]]}},"alternative-id":["10.1002\/ijc.1623"],"URL":"https:\/\/doi.org\/10.1002\/ijc.1623","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,12,29]]}}}