{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T02:45:08Z","timestamp":1791081908127,"version":"4.1.0"},"reference-count":14,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,1,22]],"date-time":"2004-01-22T00:00:00Z","timestamp":1074729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Fondo de Investigaci\u00f3n Sanitaria","award":["FIS PI020432"],"award-info":[{"award-number":["FIS PI020432"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2004,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The nuclear pore complex protein Nup88 is overexpressed in tumor cells. Immunohistochemical studies have shown that this overexpression is linked to higher aggressiveness of colorectal carcinoma and to enhanced metastatic potential of melanoma cells. However, the antibodies so far developed against Nup88 have the drawback of recognizing a number of other, up to now unspecified antigens besides Nup88. For this reason, we devised the present study on Nup88 expression at the mRNA level. RNA was extracted from fresh tumor tissue corresponding to 122 breast cancer patients. <jats:italic>Nup88<\/jats:italic> mRNA expression was measured by means of differential RT\u2010PCR, standardizing against a constitutive internal control gene <jats:italic>(\u03b2\u2010actin).<\/jats:italic> The results were dichotomized into \u201chigh\u201d and \u201clow\u201d expression levels, using the median value as cut\u2010off. High <jats:italic>Nup88<\/jats:italic> mRNA expression levels correlated significantly with ductal and tubular histology (<jats:italic>p<\/jats:italic> = 0.012), histologic and nuclear grade 3 of tumors (<jats:italic>p<\/jats:italic> &lt; 0.001), absence of hormone receptor expression (<jats:italic>p<\/jats:italic> &lt; 0.001), expression of the <jats:italic>c\u2010erb\u2010B2<\/jats:italic> oncogene (<jats:italic>p<\/jats:italic> &lt; 0.001), expression of mutant p53 protein (<jats:italic>p<\/jats:italic> &lt; 0.001), high proliferation (defined by Ki67 labeling index &gt;20%, <jats:italic>p<\/jats:italic> &lt; 0.001), DNA aneuploidy (<jats:italic>p<\/jats:italic> &lt; 0.001) as well as the most important ominous clinical prognostic factor, axillary node invasion (<jats:italic>p<\/jats:italic> &lt; 0.001). We also found an inverse correlation (<jats:italic>p<\/jats:italic> &lt; 0.001) with expression of the <jats:italic>H\u2010MAM<\/jats:italic> (mammaglobin) gene, a marker of low biologic and clinical aggressiveness of breast cancer. All of these factors, without exception, define a highly aggressive tumor phenotype. These findings appear to be specific to Nup88 and not to nuclear pore proteins in general. Indeed, analysis of Nup107 (which is a limiting component of the nuclear pore complex) under the same conditions in the same tumors did not yield comparable results. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.20034","type":"journal-article","created":{"date-parts":[[2004,3,1]],"date-time":"2004-03-01T08:02:15Z","timestamp":1078128135000},"page":"717-720","source":"Crossref","is-referenced-by-count":78,"title":["<i>Nup88<\/i> mRNA overexpression is associated with high aggressiveness of breast cancer"],"prefix":"10.1002","volume":"109","author":[{"given":"David","family":"Agudo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"G\u00f3mez\u2010Esquer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Mart\u00ednez\u2010Arribas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari\u00e1 Jos\u00e9","family":"N\u00fa\u00f1ez\u2010Villar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Poll\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Schneider","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,1,22]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"1801","article-title":"Interaction of cellular proteins with the leukemia specific fusion proteins DEK\u2010CAN and SET\u2010CAN and their normal counterpart, the nucleoporin CAN","volume":"13","author":"Fornerod M","year":"1996","journal-title":"Oncogene"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.137.5.989"},{"key":"e_1_2_6_4_2","first-page":"5408","article-title":"The nuclear pore complex protein Nup88 is overexpressed by tumor cells","volume":"59","author":"Mart\u00ednez N","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1998.167"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64798-0"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1159\/000070294"},{"key":"e_1_2_6_8_2","first-page":"43","article-title":"Expression of p16, p27, p53 and Nup88 proteins in matched primary and metastatic melanoma cells","volume":"21","author":"Hong Zhang","year":"2002","journal-title":"Int J Oncol"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6600656"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/bcr587"},{"key":"e_1_2_6_11_2","first-page":"295","article-title":"Flow cytometry vs. Ki67 labelling index in breast cancer: a prospective evaluation of 181 cases","volume":"22","author":"Martinez\u2010Arribas F","year":"2002","journal-title":"Anticancer Res"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.252749899"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb00942.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.18.3.1236"},{"key":"e_1_2_6_15_2","doi-asserted-by":"crossref","first-page":"2165","DOI":"10.1242\/jcs.115.10.2165","article-title":"Regulation, mechanisms and proposed function of ferritin translocation to cell nuclei","volume":"115","author":"Thompson KJ","year":"2002","journal-title":"J Cell Sci"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.20034","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.20034","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T23:56:41Z","timestamp":1700179001000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.20034"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1,22]]},"references-count":14,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2004,5]]}},"alternative-id":["10.1002\/ijc.20034"],"URL":"https:\/\/doi.org\/10.1002\/ijc.20034","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,1,22]]}}}