{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:08:42Z","timestamp":1770822522138,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2004,6,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Using methylation\u2010specific real\u2010time PCR, we determined the prevalence of aberrant methylation in the mismatch repair gene <jats:italic>hMLH1<\/jats:italic> and in the recently described <jats:italic>HPP1<\/jats:italic> gene among 50 esophageal, 50 cardiac and 50 gastric ADCs. Additionally, expression of hMLH1 protein was detected immunohistochemically and correlated with DNA MSI. Hypermethylation of <jats:italic>hMLH1<\/jats:italic> was found in 14% of esophageal, 28% of cardiac and 32% of gastric ADCs, whereas <jats:italic>HPP1<\/jats:italic> hypermethylation was found more frequently in the 3 tumor types (64% <jats:italic>vs.<\/jats:italic> 38% <jats:italic>vs.<\/jats:italic> 54%). In gastric ADC, <jats:italic>HPP1<\/jats:italic> hypermethylation was found more frequently in tumors with concomitant <jats:italic>hMLH1<\/jats:italic> hypermethylation (81%) than in those without <jats:italic>hMLH1<\/jats:italic> hypermethylation (41%, <jats:italic>p<\/jats:italic> = 0.008). Complete loss of hMLH1 protein expression, which was present in 10 carcinomas (5 cardiac and 5 gastric), was invariably correlated with <jats:italic>hMLH1<\/jats:italic> hypermethylation and MSI. In conclusion, our data indicate that MSI and loss of the mismatch repair protein hMLH1, which is mainly caused by <jats:italic>hMLH1<\/jats:italic> gene hypermethylation, are more prevalent in stomach and cardia carcinogenesis than in that of the esophagus. Moreover, in gastric cancer, <jats:italic>hMLH1<\/jats:italic> hypermethylation is correlated with hypermethylation of the <jats:italic>HPP1<\/jats:italic> gene. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.20058","type":"journal-article","created":{"date-parts":[[2004,3,31]],"date-time":"2004-03-31T18:01:13Z","timestamp":1080756073000},"page":"208-211","source":"Crossref","is-referenced-by-count":24,"title":["Correlation of <i>hMLH1<\/i> and <i>HPP1<\/i> hypermethylation in gastric, but not in esophageal and cardiac adenocarcinoma"],"prefix":"10.1002","volume":"110","author":[{"given":"Helene","family":"Geddert","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sibylle","family":"Kiel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erol","family":"Iskender","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea R.","family":"Florl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Krieg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Vossen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helmut E.","family":"Gabbert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"Sarbia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.33427"},{"key":"e_1_2_6_3_2","volume-title":"Classification of tumors. 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