{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T17:26:25Z","timestamp":1747243585844},"reference-count":77,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,1]],"date-time":"2005-02-01T00:00:00Z","timestamp":1107216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2005,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recent studies have shown the involvement of the Fas system (Fas receptor and its ligand FasL) in cancerous processes. The absence or downregulation of Fas, reported in the majority of human tumors, conflicts with its presence in cancerous cells from the same tumors but maintained <jats:italic>in vitro<\/jats:italic>. Recently, the eventual role of environmental factors in the loss of Fas expression, or in the <jats:italic>in vivo<\/jats:italic> selection of a Fas\u2010negative cell population has been suggested. We determined the Fas expression and function in the Capan\u20101 human cancerous pancreatic duct cells over 2 successive passages <jats:italic>in vivo<\/jats:italic> separated by a period of 10\u201320 passages <jats:italic>in vitro<\/jats:italic>. We showed that Capan\u20101 cells express Fas and are sensitive to Fas\u2010mediated apoptosis when maintained <jats:italic>in vitro<\/jats:italic>. When these cells were xenografted into nude mice the expression of Fas was lost in the majority of the tumors. Culture of tumor\u2010derived cells exhibited that they became Fas\u2010positive and sensitive to Fas\u2010mediated apoptosis after a short period <jats:italic>in vitro<\/jats:italic>. The loss\/gain of Fas was reproduced after re\u2010explantation and re\u2010culture of these Fas\u2010expressing cells. Furthermore, RT\u2010PCR evidenced a strong inhibition of Fas, FLICE and FADD mRNAs expression in the xenografts. Our observations indicate that the expression of Fas and its function could depend to factors in the tumoral environment. The <jats:italic>in vivo<\/jats:italic> loss of Fas may thus play an important role in the tumor formation and in the evasion of tumor cells from immune surveillance. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.20802","type":"journal-article","created":{"date-parts":[[2005,2,2]],"date-time":"2005-02-02T00:17:49Z","timestamp":1107303469000},"page":"214-223","source":"Crossref","is-referenced-by-count":3,"title":["Serial <i>in vivo<\/i> loss and <i>in vitro<\/i> gain of Fas expression and function in human cancerous pancreatic duct 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