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The molecular mechanism underlying this process is only partly understood but seems to require the function of connexin32 (Cx32), one of the 2 gap junction proteins expressed in hepatocytes. PB mediates transcriptional activation of various genes in liver but which of these are relevant for tumor promotion is unknown. We have used oligonucleotide microarrays to identify genes differentially modulated in expression by PB in liver of Cx32\u2010wild\u2010type and Cx32\u2010null mice. Mice of both strains were kept on PB containing (0.05%) or control diet for 2 weeks. Total liver RNA was isolated from 3 mice per experimental group and reverse transcribed; cDNAs were hybridized to oligonucleotide microarrays and a gene\u2010by\u2010gene linear model was used for statistical analysis of data. Five genes were identified as induced or repressed in untreated Cx32\u2010null as compared to untreated Cx32\u2010wild\u2010type mice. PB affected the expression of 53 genes, of which 13 code for members of Phase\u2010I\/II of drug metabolism, and 12 genes were differentially affected in expression by PB in Cx32\u2010null as compared to Cx32\u2010wild\u2010type mice. Among the differentially affected genes that could be verified by quantitative RT\u2010PCR or Western analysis were the <jats:italic>insulin like growth factor binding protein\u20101<\/jats:italic>, <jats:italic>retinol dehydrogenase\u20106<\/jats:italic> and the Y\u2010chromosomally located gene <jats:italic>Dby<\/jats:italic>, among which may be a candidate of relevance for PB\u2010mediated tumor promotion. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.20815","type":"journal-article","created":{"date-parts":[[2005,3,4]],"date-time":"2005-03-04T22:16:40Z","timestamp":1109974600000},"page":"861-869","source":"Crossref","is-referenced-by-count":10,"title":["Effect of the tumor promoter phenobarbital on the pattern of global gene expression in liver of connexin32\u2010wild\u2010type and connexin32\u2010deficient 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