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To assess tumor\u2010 and possible germline\u2010specific regions affected with gene copy number variations on this chromosome, we applied a high\u2010resolution genomic clone\u2010based chromosome 22 array to a series of 28 WT samples and the paired blood\u2010derived DNA of the patients. The group of tumors was enriched for cases with metastases, relapse or fatal outcome, criteria that were expected to yield a higher number of alterations on chromosome 22. Overall, the array\u2010based form of comparative genomic hybridization (array\u2010CGH) analysis revealed genomic changes in 53% (15 out of 28) of cases. We identified hemizygous deletion of the whole arm of 22q in 3 tumors (11%). Furthermore, a complex amplifier genotype was detected in 8 samples, presenting regions of gain along the chromosome, which defined 7 distinct minimal overlapping segments. The distribution of aberrations in 4 additional cases displaying regional genomic imbalances delimited 2 tumor suppressor\/oncogene candidate loci, 1 in the proximal and the other in the terminal part of 22q. Analysis of these regions revealed the presence of several candidate genes that may play a role in the development of WT. These findings demonstrate the power of array\u2010CGH in the determination of DNA copy number alterations and further strength the notion that WT\u2010associated genes exist on this chromosome. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.21868","type":"journal-article","created":{"date-parts":[[2006,2,23]],"date-time":"2006-02-23T00:17:20Z","timestamp":1140653840000},"page":"571-578","source":"Crossref","is-referenced-by-count":8,"title":["Identification of limited regions of genetic aberrations in patients affected with Wilms' tumor using a tiling\u2010path chromosome 22 array"],"prefix":"10.1002","volume":"119","author":[{"given":"Magdalena","family":"Benetkiewicz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresita D\u00edaz","family":"de 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