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To identify such targets, we screened cDNA libraries from human testis, lung and breast cancer, bovine and porcine muscle and wheat germ for reactivity with paraproteins in the sera from 115 patients with MGUS and MM. Of &gt;6 \u00d7 10<jats:sup>8<\/jats:sup> paraprotein\u2013antigen interactions screened, an IgA paraprotein from a female patient bound to sperm\u2010specific cylicin\u20102, and 3 IgG paraproteins bound to tripeptidyl\u2010peptidase\u2010II (TPP\u20102), insulin\u2010like growth\u2010factor binding\u2010protein\u20102 (IGFBP\u20102) and porcine kinesin. Specificity was confirmed by reverse Western blots using recombinant antigens. The broad spectrum of auto\u2010, allo\u2010 and heteroantigens as targets of human paraproteins in patients without signs of chronic antigenic stimulation renders a causal role of the antigenic stimulus in the pathogenesis of MGUS and MM unlikely. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.22686","type":"journal-article","created":{"date-parts":[[2007,3,20]],"date-time":"2007-03-20T19:31:37Z","timestamp":1174419097000},"page":"459-461","source":"Crossref","is-referenced-by-count":11,"title":["Identification of antigenic targets of paraproteins by expression cloning does not support a causal role of chronic antigenic stimulation in the pathogenesis of multiple myeloma and MGUS"],"prefix":"10.1002","volume":"121","author":[{"given":"Klaus\u2010Dieter","family":"Preuss","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"Held","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris","family":"Kubuschok","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun\u2010Zhu","family":"Hung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Malatsidze","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias","family":"Wagner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Pfreundschuh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,5,18]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.18.8482"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.174.3.525"},{"key":"e_1_2_5_4_2","first-page":"163","article-title":"Antibody 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