{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T15:34:10Z","timestamp":1758814450037},"reference-count":41,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2007,8,20]],"date-time":"2007-08-20T00:00:00Z","timestamp":1187568000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2007,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The association between total and integrated HPV\u201033 DNA loads and high\u2010grade squamous intraepithelial lesions (HSIL) of the uterine cervix was investigated. Of 5,347 women recruited in 4 studies, 89 (64 without SIL, 7 low\u2010grade SIL (LSIL), 15 HSIL, 3 unknown grade) were infected by HPV\u201033. HPV\u201033 E6, HPV\u201033 E2 and \u03b2\u2010globin DNA were measured with real\u2010time PCR that allowed to assess total (E6), episomal (E2) and integrated (E6\u2013E2) HPV\u201033 viral loads. HPV\u201033 E6\/E2 ratios \u2265\u22652.0 suggesting the presence of integrated HPV\u201033 were obtained for 28.6% (<jats:italic>n<\/jats:italic> = 18) of women without SIL and 21.4% (<jats:italic>n<\/jats:italic> = 3) of women with HSIL (<jats:italic>p<\/jats:italic> = 0.74). Although median viral loads were similar, there was a trend toward having a greater proportion of women with HSIL in the fourth quartile (\u2265\u226510<jats:sup>6.69<\/jats:sup> copies\/\u03bcg DNA) of total HPV\u201033 viral loads compared to normal women. Controlling for age, site, ethnicity and LCR polymorphism by logistic regression, HPV\u201033 total loads in the fourth quartile {odds ratio (OR) 4.5 [95% confidence interval (CI) 1.2\u201317.3]; <jats:italic>p<\/jats:italic> <jats:italic>=<\/jats:italic> 0.03} and episomal loads in the fourth quartile (\u2265\u226510<jats:sup>6.64<\/jats:sup> copies\/\u03bcg DNA) [OR 3.9 (95% CI 1.1\u201313.2); <jats:italic>p<\/jats:italic> = 0.05] but not integrated HPV\u201033 load in the fourth quartile [OR 1.0 (95% CI 0.3\u20133.3); <jats:italic>p<\/jats:italic> = 0.50] were associated with HSIL. Controlling for age, study site and SIL grade, HPV\u201033 episomal load [OR 0.2 (95% CI 0.1\u20130.5), <jats:italic>p<\/jats:italic> = 0.0004] was associated with the presence of HPV\u201033 integration. High episomal loads in HSIL and the presence of integration in women without SIL are likely to weaken the usefulness of HPV load of integrated forms in clinical practice. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.23006","type":"journal-article","created":{"date-parts":[[2007,8,20]],"date-time":"2007-08-20T23:51:24Z","timestamp":1187653884000},"page":"2674-2681","source":"Crossref","is-referenced-by-count":14,"title":["Viral load of episomal and integrated forms of human papillomavirus type 33 in high\u2010grade squamous intraepithelial lesions of the uterine cervix"],"prefix":"10.1002","volume":"121","author":[{"given":"Soraya","family":"Khouadri","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luisa L.","family":"Villa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon","family":"Gagnon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anita","family":"Koushik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harriet","family":"Richardson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Greg","family":"Matlashewski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel","family":"Roger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex S.","family":"Ferenczy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo L.","family":"Franco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois","family":"Coutl\u00e9e","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,8,20]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.55.4.244"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(01)00617-9"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.aids.0000168972.65304.6b"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10846"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02402-8"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6602436"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jncimonographs.a003480"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/509622"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800737"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1490"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.5.1755-1762.2006"},{"key":"e_1_2_7_13_2","first-page":"6132","article-title":"Detection of high\u2010risk cervical intraepithelial neoplasia and cervical cancer by amplification of transcripts derived from integrated papillomavirus oncogenes","volume":"59","author":"Klaes R","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2004.024570"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.3.886-891.2002"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.56.7.532"},{"key":"e_1_2_7_17_2","first-page":"959","article-title":"Optimization and validation of a multiplexed luminex assay to quantify antibodies to neutralizing epitopes on human papillomaviruses 6, 11, 16 and 18","volume":"12","author":"Dias D","year":"2005","journal-title":"Clin Diagn Lab Immunol"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2004.03.033"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.05.026"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6601024"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-05-0055"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1086\/507431"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cdp.2005.06.007"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.286.24.3106"},{"key":"e_1_2_7_25_2","first-page":"485","article-title":"The natural history of type\u2010specific HPV infections in female university students","volume":"12","author":"Richardson H","year":"2003","journal-title":"Cancer Epidemiol Biomarkers Prev"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2003.66"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.58.3.991-995.1986"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2003.09.003"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.10539"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/3327"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.mlg.0000147918.81733.49"},{"key":"e_1_2_7_32_2","first-page":"5916","article-title":"Human papillomavirus type 16 integrations in cervical tumors frequently occur in common fragile sites","volume":"60","author":"Thorland EC","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1086\/431978"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20480"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21956"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.6.2763-2767.2003"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206629"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004347-199804000-00009"},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.64.2.813-821.1990"},{"key":"e_1_2_7_40_2","doi-asserted-by":"publisher","DOI":"10.1054\/bjoc.2001.1695"},{"key":"e_1_2_7_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2006.09.008"},{"key":"e_1_2_7_42_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20894"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.23006","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.23006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T09:44:16Z","timestamp":1696844656000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.23006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,8,20]]},"references-count":41,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2007,12,15]]}},"alternative-id":["10.1002\/ijc.23006"],"URL":"https:\/\/doi.org\/10.1002\/ijc.23006","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,8,20]]}}}