{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T23:39:21Z","timestamp":1790552361120,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2007,8,23]],"date-time":"2007-08-23T00:00:00Z","timestamp":1187827200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2007,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cervicovaginal human papillomavirus (HPV) viral load has been purported as a potential marker for the detection of high\u2010grade cervical intraepithelial neoplasia or cancer (\u2265CIN2). To examine disease association with type\u2010specific viral load for the full\u2010range of anogenital HPV infections, we conducted cross\u2010sectional and prospective analyses of \u223c2,000 HPV\u2010infected women from a 10,000\u2010woman population\u2010based study in Guanacaste, Costa Rica with 7 years of follow\u2010up. Cervical specimens were tested for &gt;40 HPV types using a MY09\/MY11 L1 consensus primer PCR method with type\u2010specific dot blot hybridization and PCR signal intensity as a measure of viral load. A positive association was observed between prevalent \u2265CIN2 and high viral load compared to low viral load for women with baseline single HPV16 infections (OR = 19.2, 95% CI = 4.4\u201383.2) and single non\u201016 carcinogenic infections (OR = 9.2, 95% CI = 2.1\u201339.9). Inclusion of women with multiple HPV types did not substantially change these associations. In prospective follow\u2010up, only women infected with HPV16 alone (OR = 27.2, 95% = 3.5\u2013213.5) had a strong association between high viral load and incident \u2265CIN2; non\u201016 carcinogenic high viral load was not associated with incident \u2265CIN2 (OR = 0.7, 95% CI = 0.2\u20131.9). Single noncarcinogenic type viral load was not associated with increased risk of prevalent or incident \u2265CIN2 (OR = 1.2 and 1.1, respectively). In conclusion, carcinogenic high viral load was associated with prevalent \u2265CIN2; however HPV16 was uniquely associated with incident \u2265CIN2. The extent to which these observations can be translated into clinical practice must be rigorously examined in the context of the method of viral load measurement and the type\u2010specific differences observed for incident \u2265CIN2. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.23012","type":"journal-article","created":{"date-parts":[[2007,8,25]],"date-time":"2007-08-25T01:54:31Z","timestamp":1188006871000},"page":"2787-2793","source":"Crossref","is-referenced-by-count":134,"title":["High load for most high risk human papillomavirus genotypes is associated with prevalent cervical cancer precursors but only HPV16 load predicts the development of incident disease"],"prefix":"10.1002","volume":"121","author":[{"given":"Patti E.","family":"Gravitt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melinda 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