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Using vitamin C\u2010deficient B6.129P2\u2010Gulo<jats:sup><jats:italic>tm1Umc<\/jats:italic>\/mmcd<\/jats:sup> (gulo<jats:sup>\u2212\/\u2212<\/jats:sup>) mice lacking <jats:sc>L<\/jats:sc>\u2010gulono\u2010\u03b3\u2010lactone oxidase, we compared gastric lesions and Th1 immune responses in <jats:italic>H. pylori<\/jats:italic>\u2010infected gulo<jats:sup>\u2212\/\u2212<\/jats:sup> mice supplemented with low (33 mg\/L) or high (3,300 mg\/L) vitamin C in drinking water for 16 or 32 weeks. Vitamin C levels in plasma and gastric tissue correlated with the vitamin C supplementation levels in gulo<jats:sup>\u2212\/\u2212<\/jats:sup> mice. <jats:italic>H. pylori<\/jats:italic> infection resulted in comparable gastritis and premalignant lesions in wildtype C57BL\/6 and gulo<jats:sup>\u2212\/\u2212<\/jats:sup> mice supplemented with high vitamin C, but lesions were less severe in gulo<jats:sup>\u2212\/\u2212<\/jats:sup> mice supplemented with low vitamin C at 32 weeks post infection. The reduced gastric lesions in infected gulo<jats:sup>\u2212\/\u2212<\/jats:sup> mice supplemented with low vitamin C correlated with reduced Th1\u2010associated IgG2c, gastric IFN\u2010\u03b3 and TNF\u2010\u03b1 mRNA and higher <jats:italic>H. pylori<\/jats:italic> colonization levels. These results in the <jats:italic>H. pylori<\/jats:italic>\u2010infected gulo<jats:sup>\u2212\/\u2212<\/jats:sup> mouse model suggest that although supplementation with a high level of vitamin C achieved physiologically normal vitamin C levels in plasma and gastric tissue, this dose of vitamin C did not protect gulo<jats:sup>\u2212\/\u2212<\/jats:sup> mice from <jats:italic>H. pylori<\/jats:italic>\u2010induced premalignant gastric lesions. In addition, less severe gastric lesions in <jats:italic>H.pylori<\/jats:italic> infected gulo<jats:sup>\u2212\/\u2212<\/jats:sup> mice supplemented with low vitamin C correlated with an attenuated Th1 inflammatory response. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.23228","type":"journal-article","created":{"date-parts":[[2007,11,7]],"date-time":"2007-11-07T20:18:28Z","timestamp":1194466708000},"page":"1068-1076","source":"Crossref","is-referenced-by-count":20,"title":["Vitamin C supplementation does not protect <scp>L<\/scp>\u2010gulono\u2010\u03b3\u2010lactone oxidase\u2010deficient mice from <i>Helicobacter pylori<\/i>\u2010induced gastritis and gastric premalignancy"],"prefix":"10.1002","volume":"122","author":[{"given":"Chung\u2010Wei","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang\u2010Dong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuo\u2010Liong","family":"Chien","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongming","family":"Ge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barry H.","family":"Rickman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arlin B.","family":"Rogers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Varro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark 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