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The role of FH in colon cancer has not yet been examined. Here, we studied immunocytochemically FH\/FHL expression in tumor samples derived from 40 patients, with both primary colon adenocarcinoma and metastatic foci in the liver. FH\/FHL immunoreactivity was present in stroma of both primary and metastatic tumors, in virtually all patients. The cellular immunoreactivity was observed infrequently. Importantly, when analyzed quantitatively, FH\/FHL immunoreactivity was significantly increased in liver metastases when compared with the primary sites. In addition, we have analyzed FH and FHL expression in 5 colon cancer cell lines: SW480, SW620, HCT116, HT\u201029 and Lovo. FH mRNA and FH secretion were observed in SW620 and HT\u201029 cells, whereas FHL was produced only by HT\u201029 cell\u2010line. By confocal and electron microscopy, FH immunoreactivity was associated with the plasma membrane and intracellular vesicular structures. Finally, we have analyzed the role of FH in the susceptibility of SW620 colon cancer cells to complement\u2010mediated damage. When FH function was blocked, using specific antibody, the cells became more susceptible to lysis. Taken together, our results suggest an important role of FH\/FHL in colon cancer cells defense against complement\u2010mediated cytotoxicity, and in metastatic process. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.23238","type":"journal-article","created":{"date-parts":[[2008,1,8]],"date-time":"2008-01-08T18:16:35Z","timestamp":1199816195000},"page":"2030-2037","source":"Crossref","is-referenced-by-count":53,"title":["The possible role of factor H in colon cancer resistance to complement 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