{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:56:46Z","timestamp":1791194206805,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2008,3,6]],"date-time":"2008-03-06T00:00:00Z","timestamp":1204761600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2008,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Staging by sentinel node (SN) biopsy is the standard procedure for clinically node\u2010negative breast cancer patients. Intra\u2010operative analysis of the SN allows immediate axillary lymph node (ALN) dissection in SN positive patients, but a quick, reliable and reproducible method is lacking. We tested the suitability of a quantitative cytokeratin 19 (CK19) mRNA one step nucleic acid amplification (OSNA<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"#note1\">#<\/jats:ext-link>) technique (OSNA\u2010CK19) for intra\u2010operative SN analysis. OSNA\u2010CK19 involves a short manual sample preparation step and subsequent fully automated amplification of CK19 mRNA based on reverse transcription loop\u2010mediated isothermal amplification, with results available within 30\u201340 min. OSNA\u2010CK19 was compared to histological staining (Hematoxylin&amp;Eosin and CAM5.2 and CK19 immunostaining) of 346 frozen ALNs from 32 breast cancer patients, using half of the lymph node for each method. 267 samples were negative and 61 positive by both methods. Three samples were histology positive and OSNA\u2010CK19 negative. Fifteen samples were histology negative and OSNA\u2010CK19 positive, 11 of which had copy numbers close to the cut\u2010off level of OSNA\u2010CK19. Seven of these 15 samples were RT\u2010PCR positive for epithelial markers and\/or showed CK19 protein expression by Western blot suggesting the presence of tumor deposits in the lymph node part investigated by OSNA\u2010CK19. Concordance with histology was 94.8%, and 96.8% after exclusion of the latter 7 discordant cases. Sensitivity was 95.3% and specificity was 94.7% before and 97.1% after discordant case investigation. Our results indicate that OSNA\u2010CK19 can potentially be useful in an intra\u2010operative clinical setting to detect SN tumor involvement in breast cancer patients. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.23451","type":"journal-article","created":{"date-parts":[[2008,3,6]],"date-time":"2008-03-06T17:10:43Z","timestamp":1204823443000},"page":"2562-2567","source":"Crossref","is-referenced-by-count":150,"title":["Intra\u2010operative rapid diagnostic method based on CK19 mRNA expression for the detection of lymph node metastases in breast 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