{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T01:23:56Z","timestamp":1790817836604,"version":"4.1.0"},"reference-count":62,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,4,15]],"date-time":"2008-04-15T00:00:00Z","timestamp":1208217600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The E6 and E7 proteins of human papillomaviruses (HPV) play a crucial role in the pathogenesis of malignant tumors. E6 protein of high\u2010risk mucosal papillomaviruses targets a number of proteins for proteosomal degradation through complex formation with ubiquitin ligase E6AP. These proteins include, amongst others, p53, paxillin and PDZ\u2010domain proteins <jats:italic>e.g<\/jats:italic>. Dlg and MAGUK. The mechanism by which the E6 protein of cutaneous HPV types interacts with cellular proteins to induce either benign or malignant cutaneous lesions, has not been elucidated, although extensive ultraviolet exposure and mutated p53 (<jats:italic>hot\u2010spot<\/jats:italic> mutations) are known to be associated with non\u2010melanoma skin cancer. We demonstrate two mechanisms in which HPV20E6 may be involved in the infected cell. One pathway is the wtp53 mediated degradation of HPV20E6 through caspase\u20103. Mutated p53R248W and \u0394Np63\u03b1, as well as other unknown proteins involved in proteosome\u2010dependent degradation, convey a protective effect on HPV20E6 under these conditions. This unveils a remarkable opposite regulation to the well\u2010known mechanism of E6\u2010E6AP mediated degradation of p53 for mucosal HPV types. In a second interaction, ectopically expressed HPV20E6 induces cleavage of procaspase\u20103 to active caspase\u20103. We demonstrate, in addition, <jats:italic>in vivo<\/jats:italic> binding of HPV20E6 to the intermediate filament vimentin. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.23506","type":"journal-article","created":{"date-parts":[[2008,4,15]],"date-time":"2008-04-15T22:13:35Z","timestamp":1208297615000},"page":"108-116","source":"Crossref","is-referenced-by-count":12,"title":["Degradation of HPV20E6 by p53: \u0394Np63\u03b1 and mutant p53R248W protect the wild type p53 mediated caspase\u2010degradation"],"prefix":"10.1002","volume":"123","author":[{"given":"Jian\u2010Wei","family":"Fei","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ethel\u2010Michele","family":"de Villiers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,4,24]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"F55","article-title":"Papillomavirus infections\u2014a major cause of human cancers","volume":"1288","author":"zur Hausen H","year":"1996","journal-title":"Biochim Biophys Acta"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc798"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1991.tb04990.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.13.8.4918"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204869"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1203374"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M605117200"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-78487-3_9"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0753-3322(97)86238-5"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1998.00191.x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.24.11636-11641.2000"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1086\/521031"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.9.4216"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206026"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.182100"},{"key":"e_1_2_6_17_2","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1126\/science.281.5381.1312","article-title":"Caspases: enemies within","volume":"28","author":"Thornberry NA","year":"1998","journal-title":"Science"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(03)00497-0"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M313683200"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(04)00098-X"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4402047"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0707947104"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1997"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4402038"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1208201"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb0607-621"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1597"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4402060"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.10.7337"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00739"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.4161\/cc.3.12.1318"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.23.13664"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2004.02040.x"},{"key":"e_1_2_6_34_2","first-page":"4386","article-title":"Caspase\u20103 is essential for procaspase\u20109 processing and cisplatin\u2010induced apoptosis of MCF\u20107 breast cancer cells","volume":"60","author":"Blanc C","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1115035"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1210773"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1006\/scbi.1999.0145"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00954-06"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.4161\/cc.4.5.1653"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209420"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0700761104"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4400840"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.27.17102"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/24.4.596"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth1204-275"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm731"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1127085"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90384-3"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2006.08.038"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.22.10124"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.19679-0"},{"key":"e_1_2_6_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/35078527"},{"key":"e_1_2_6_53_2","first-page":"6660","article-title":"APAF\u20101 is a transcriptional target of p53 in DNA damage\u2010induced apoptosis","volume":"61","author":"Robles AI","year":"2001","journal-title":"Cancer Res"},{"key":"e_1_2_6_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.22.24.8601-8611.2002"},{"key":"e_1_2_6_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1535-6108(02)00064-8"},{"key":"e_1_2_6_56_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C100025200"},{"key":"e_1_2_6_57_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.52.33373"},{"key":"e_1_2_6_58_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1201504"},{"key":"e_1_2_6_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2007.03.040"},{"key":"e_1_2_6_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.7.4509-4516.1996"},{"key":"e_1_2_6_61_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1210311"},{"key":"e_1_2_6_62_2","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.371.2.7"},{"key":"e_1_2_6_63_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.9.4412"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.23506","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.23506","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T14:49:04Z","timestamp":1695912544000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.23506"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,4,24]]},"references-count":62,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,7]]}},"alternative-id":["10.1002\/ijc.23506"],"URL":"https:\/\/doi.org\/10.1002\/ijc.23506","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,4,24]]}}}