{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T22:29:28Z","timestamp":1790980168983,"version":"4.1.0"},"reference-count":55,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2008,8,7]],"date-time":"2008-08-07T00:00:00Z","timestamp":1218067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2008,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Penile carcinomas are frequently associated with high risk human papillomavirus (HPV) types. Because little is known about the molecular biology of this association, we investigated three properties of HPV genomes in penile carcinomas from Brazilian patients: (<jats:italic>i<\/jats:italic>) HPV DNA methylation, (<jats:italic>ii<\/jats:italic>) junctions between HPV and cellular DNA and (<jats:italic>iii<\/jats:italic>) genomic variation. In cervical carcinogenesis, recombination between HPV and chromosomal DNA is frequent and likely necessary for progression, and DNA hypermethylation\u2014specifically of the <jats:italic>L1<\/jats:italic> gene\u2014is a biomarker for cancerous progression. The same mechanisms apparently occur during penile carcinogenesis, because 95 HPV\u201016 molecules derived from 19 penile lesions had 58% of the CpGs in <jats:italic>L1<\/jats:italic> and 22% in the 5\u2032 part of the long control region methylated, more than the percentages found in cervical carcinomas. In addition, 2 out of 3 HPV\u201018 infections, all present in double infections with HPV\u201016, showed <jats:italic>L1<\/jats:italic> specific methylation typical of malignant cervical lesions. In 11 out of 15 HPV\u201016 lesions, we confirmed chromosomal integration by reverse ligation inverted PCR, while 4 samples had concatemeric integrations or episomes. Nine of 17 penile carcinomas contained HPV\u201016 AA variants, and 8 E variants. As AA variants are relatively rare in Brazilian cohorts of asymptomatic women, the high prevalence in penile carcinomas may indicate a higher risk of progression of AA lesions, as suspected for cervical infections. Our observations of frequent viral DNA methylation, chromosomal integration and the prevalence of high risk variants suggest that HPV\u2010dependent carcinogenesis of the penis and cervix follows similar etiological and epidemiological parameters. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.23707","type":"journal-article","created":{"date-parts":[[2008,8,7]],"date-time":"2008-08-07T19:34:46Z","timestamp":1218137686000},"page":"1832-1840","source":"Crossref","is-referenced-by-count":53,"title":["Human papillomavirus\u201016 and \u201018 in penile carcinomas: DNA methylation, chromosomal recombination and genomic variation"],"prefix":"10.1002","volume":"123","author":[{"given":"Mina","family":"Kalantari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luisa L.","family":"Villa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Itzel E.","family":"Calleja\u2010Macias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans\u2010Ulrich","family":"Bernard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,8,13]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"247","article-title":"Penile cancer. 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