{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T16:33:37Z","timestamp":1787502817835,"version":"build-2736575974"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,9,26]],"date-time":"2008-09-26T00:00:00Z","timestamp":1222387200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2009,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    To investigate the relationship between\n                    <jats:italic>p16<\/jats:italic>\n                    methylation and\n                    <jats:italic>Helicobacter pylori<\/jats:italic>\n                    infection in precancerous gastric lesions, a population\u2010based study was conducted in Linqu County, a high\u2010risk area of gastric cancer in China. Methylation status of\n                    <jats:italic>p16<\/jats:italic>\n                    was evaluated by methylation\u2010specific polymerase chain reaction in 920 subjects with precancerous gastric lesions.\n                    <jats:italic>H. pylori<\/jats:italic>\n                    status was determined by\n                    <jats:sup>13<\/jats:sup>\n                    C\u2010urea breath test and the density of\n                    <jats:italic>H. pylori<\/jats:italic>\n                    in biopsy specimens used for detecting methylation status was assessed by the modified Giemsa stain. The frequency of\n                    <jats:italic>p16<\/jats:italic>\n                    methylation was significantly higher in subjects with\n                    <jats:italic>H. pylori<\/jats:italic>\n                    positive than those with\n                    <jats:italic>H. pylori<\/jats:italic>\n                    negative in each category of gastric lesion (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.001, respectively). Compared with\n                    <jats:italic>H. pylori<\/jats:italic>\n                    negative, the odds ratios (ORs) of\n                    <jats:italic>p16<\/jats:italic>\n                    methylation were markedly elevated in subjects with\n                    <jats:italic>H. pylori<\/jats:italic>\n                    positive for superficial gastritis (OR, 9.45; 95% confidence interval [CI]: 2.94\u201330.41), chronic atrophic gastritis (OR, 15.92; 95%CI: 7.60\u201333.36), intestinal metaplasia (OR, 4.46; 95%CI: 2.44\u20138.13), indefinite dysplasia (OR, 3.67; 95%CI: 1.90\u20137.10), and dysplasia (OR, 2.48; 95%CI: 1.02\u20135.99). Moreover, the frequencies of\n                    <jats:italic>p16<\/jats:italic>\n                    methylation increased steadily with the severity of\n                    <jats:italic>H. pylori<\/jats:italic>\n                    density in gastric mucosa. Compared with\n                    <jats:italic>H. pylori<\/jats:italic>\n                    negative, the OR of\n                    <jats:italic>p16<\/jats:italic>\n                    methylation was 1.02\u201316.13 times higher in subjects with mild\n                    <jats:italic>H. pylori<\/jats:italic>\n                    infection, and 2.69\u201338.73 times higher in those with moderate\/severe infection, respectively. Our findings indicate that\n                    <jats:italic>p16<\/jats:italic>\n                    methylation was significantly associated with\n                    <jats:italic>H. pylori<\/jats:italic>\n                    infection in precancerous gastric lesions, suggesting that\n                    <jats:italic>H. pylori<\/jats:italic>\n                    infection could potently induce methylation of\n                    <jats:italic>p16<\/jats:italic>\n                    CpG island. \u00a9 2008 Wiley\u2010Liss, Inc.\n                  <\/jats:p>","DOI":"10.1002\/ijc.23891","type":"journal-article","created":{"date-parts":[[2008,9,26]],"date-time":"2008-09-26T15:25:15Z","timestamp":1222442715000},"page":"434-439","source":"Crossref","is-referenced-by-count":48,"title":["Promoter methylation of\n                    <i>p16<\/i>\n                    associated with\n                    <i>Helicobacter pylori<\/i>\n                    infection in precancerous gastric lesions: A 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