{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T23:39:23Z","timestamp":1790552363419,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,11,11]],"date-time":"2008-11-11T00:00:00Z","timestamp":1226361600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2009,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In a population\u2010based cervical screening cohort, we determined the value of type\u2010specific viral load assessment for the detection of high\u2010grade cervical intraepithelial neoplasia and cervical cancer (\u2265CIN2). Viral load was determined by type\u2010specific real\u2010time PCR in women with single HPV16,\u201018,\u201031 and \u201033 infections, as determined by GP5+\/6+\u2010PCR. Study endpoints were the detection of cumulative \u2265CIN2 or \u2265CIN3 within 18 months of follow\u2010up. High viral loads of HPV16,\u201031, and \u201033 were predictive for \u2265CIN2 (relative risk of 1.6 (95% CI: 1.3\u20131.9), 1.7 (95% CI: 1.1\u20132.7) and 1.9 (95% CI: 1.1\u20133.1) per 10\u2010fold change in viral load, respectively). For HPV18, the relative risk was of similar magnitude (1.5, 95% CI: 0.7\u20133.1), though not significant (<jats:italic>p<\/jats:italic> = 0.3). Subsequently, we determined the sensitivities of viral load for \u2265CIN2 and \u2265CIN3 in HPV DNA\u2010positive women using viral load thresholds previously defined in a cross\u2010sectional study. These thresholds were based on the 25th, 33rd and 50th percentiles of type\u2010specific HPV16,\u201018,\u201031 or \u201033 viral load values found in women with normal cytology. For all types, combined sensitivities for \u2265CIN2 were 93.5%, 88.8% and 77.7% for the 25th, 33rd and 50th percentile thresholds, respectively. Response\u2010operator\u2010characteristics (ROC) curve analysis showed that viral load testing on HPV DNA\u2010positive women in addition to or instead of cytology may result in an increased sensitivity for \u2265CIN2, but at the cost of a marked decrease in specificity in relation to cytology. Similar results were obtained when using \u2265CIN3 as endpoint. In conclusion, in a cervical screening setting viral load assessment of HPV16, 18, 31 and 33 has no additive value to stratify high\u2010risk HPV GP5+\/6+\u2010PCR\u2010positive women for risk of \u2265CIN2 or \u2265CIN3. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.23940","type":"journal-article","created":{"date-parts":[[2008,11,11]],"date-time":"2008-11-11T20:02:50Z","timestamp":1226433770000},"page":"381-386","source":"Crossref","is-referenced-by-count":56,"title":["High\u2010risk human papillomavirus DNA load in a population\u2010based cervical screening cohort in relation to the detection of high\u2010grade cervical intraepithelial neoplasia and cervical cancer"],"prefix":"10.1002","volume":"124","author":[{"given":"Albertus 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