{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:09:50Z","timestamp":1790669390533,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2009,1,22]],"date-time":"2009-01-22T00:00:00Z","timestamp":1232582400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2009,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>CT45 is a cancer\/testis gene that we previously identified by massively parallel signature sequencing. Encoded by a multigene family on chromosome X, CT45 showed restricted mRNA expression to normal testis and various cancers. In this study, monoclonal antibodies were generated against recombinant CT45 protein, and CT45 protein expression in normal and tumor tissues was evaluated by immunohistochemical analysis. In adult normal tissue, CT45 expression was restricted to testicular germ cells, detected as a nuclear protein mainly at the stage of primary spermatocytes. In tumors, CT45 protein expression correlated with the mRNA levels detected by quantitative RT\u2010PCR, and most lung cancer and ovarian cancers with CT45 mRNA at levels &gt;1% of testicular expression were CT45 protein\u2010positive. In positive cases, CT45 showed expression patterns that ranged from diffuse strong staining to heterogeneous and patchy expression. In lung cancer, CT45 expression was least frequent in adenocarcinoma, more frequent in squamous cell carcinoma and neuroendocrine tumors. Using tissue microarrays, 376 lung cancer, 219 ovarian cancer and 155 breast cancer were evaluated for CT45 protein expression. The expression frequency was highest in ovarian cancer (37%), followed by lung cancer (13%) and lowest in breast cancer (&lt;5%). Given the focal nature of CT45 expression in many cases, these numbers represented the minimal frequency of expression in these tumor types. In summary, the expression frequency and characteristics of CT45 expression are similar to other CT cancer vaccine targets currently in clinical trials, <jats:italic>e.g<\/jats:italic>., NY\u2010ESO\u20101 and MAGE\u2010A, suggesting CT45 as a potentially useful cancer target. \u00a9 2009 UICC<\/jats:p>","DOI":"10.1002\/ijc.24296","type":"journal-article","created":{"date-parts":[[2009,1,22]],"date-time":"2009-01-22T20:10:37Z","timestamp":1232655037000},"page":"2893-2898","source":"Crossref","is-referenced-by-count":51,"title":["Cancer\/testis antigen CT45: Analysis of mRNA and protein expression in human cancer"],"prefix":"10.1002","volume":"124","author":[{"given":"Yao\u2010Tseng","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melinda","family":"Hsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peishan","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra J.","family":"Shin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulette","family":"Mhawech\u2010Fauceglia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunle","family":"Odunsi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasser K.","family":"Altorki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao\u2010Jun","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo\u2010Quan","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Simpson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lloyd J.","family":"Old","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,3,18]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.3.725"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(20000301)85:5<726::AID-IJC21>3.0.CO;2-F"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.179.3.921"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1840703"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1133324100"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.2.265"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.8.1349"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.8.1163"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(02)00479-3"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21264"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.220413497"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0606512103"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.20.10917"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(06)95001-5"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0403572101"},{"key":"e_1_2_5_17_2","first-page":"6076","article-title":"NY\u2010ESO\u20101 and LAGE\u20101 cancer\u2010testis antigens are potential targets for immunotherapy in epithelial ovarian cancer","volume":"63","author":"Odunsi K","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_5_18_2","first-page":"9","article-title":"T cell immunomonitoring and tumor responses in patients immunized with a complex of cholesterol\u2010bearing hydrophobized pullulan (CHP) and NY\u2010ESO\u20101 protein","volume":"7","author":"Uenaka A","year":"2007","journal-title":"Cancer Immun"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0502583102"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20298"},{"key":"e_1_2_5_21_2","first-page":"9","article-title":"Identification of CT46\/HORMAD1, an immunogenic cancer\/testis antigen encoding a putative meiosis\u2010related protein","volume":"5","author":"Chen YT","year":"2005","journal-title":"Cancer Immun"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.3.1004"},{"key":"e_1_2_5_23_2","first-page":"7","article-title":"Generation of monoclonal antibodies to cancer\/testis (CT) antigen CT10\/MAGE\u2010C2","volume":"6","author":"Zhuang R","year":"2006","journal-title":"Cancer Immun"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.5.1914"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-065X.2002.18803.x"},{"key":"e_1_2_5_26_2","first-page":"1","article-title":"The cancer\/testis genes: review, standardization, and commentary","volume":"4","author":"Scanlan MJ","year":"2004","journal-title":"Cancer Immun"},{"key":"e_1_2_5_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1669"},{"key":"e_1_2_5_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.1282"},{"key":"e_1_2_5_29_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-05-1203"},{"key":"e_1_2_5_30_2","first-page":"57","article-title":"Quantitative real\u2010time RT\u2010PCR analysis of NY\u2010ESO\u20101 and LAGE\u20101a mRNA expression in normal tissues and tumors, and correlation of the protein expression with the mRNA copy number","volume":"26","author":"Sato S","year":"2005","journal-title":"Int J Oncol"},{"key":"e_1_2_5_31_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-04-0819"},{"key":"e_1_2_5_32_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-0186"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.24296","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.24296","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T07:27:00Z","timestamp":1696836420000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.24296"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,3,18]]},"references-count":31,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2009,6,15]]}},"alternative-id":["10.1002\/ijc.24296"],"URL":"https:\/\/doi.org\/10.1002\/ijc.24296","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,3,18]]}}}