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In this report, we show that 3 anti\u2010endoglin (ENG) monoclonal antibodies (mAbs) SN6a, SN6j and SN6k which define individually distinct epitopes of ENG of tumor vasculature are capable of suppressing tumor metastases in the multiple metastasis models. The metastasis models were generated by i.v., s.c. (into flank) or mammary gland fat pad injection of 4T1 murine mammary carcinoma cells and splenic injection of two types of colon26 murine colorectal carcinoma cells. Individual mAbs were injected i.v. <jats:italic>via<\/jats:italic> the tail vein of mice. SN6a and SN6j effectively suppressed the formation of metastatic colonies of 4T1 in the lung in all of the three 4T1 metastatic models. In addition, these mAbs were effective for suppressing the primary tumors of 4T1 in the skin and mammary fat pad. These mAbs effectively suppressed microvessel density and angiogenesis in tumors as measured by the Matrigel plug assay in mice. No significant side effects of the administered mAbs were detected. Furthermore, SN6a and SN6j extended survival of the tumor\u2010bearing mice. SN6j, SN6k and their immunoconjugates with deglycosylated ricin A\u2010chain were all effective for suppressing hepatic metastasis of colon26. The findings in the present study are clinically relevant in view of the ongoing clinical trial of a humanized (chimerized) form of SN6j. \u00a9 2009 UICC<\/jats:p>","DOI":"10.1002\/ijc.24482","type":"journal-article","created":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T19:38:25Z","timestamp":1239046705000},"page":"1446-1453","source":"Crossref","is-referenced-by-count":67,"title":["Anti\u2010endoglin monoclonal antibodies are effective for suppressing metastasis and the primary tumors by targeting tumor 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