{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T19:20:36Z","timestamp":1790277636285,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,4,7]],"date-time":"2009-04-07T00:00:00Z","timestamp":1239062400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2009,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The cell\u2013cell adhesion protein junctional adhesion molecule\u2010A (JAM\u2010A) influences epithelial cell morphology and migration. As migration is required for tumor cell invasion and metastasis, we sought to elucidate the role of JAM\u2010A in invasive breast cancer. A breast cancer tissue microarray was analyzed for JAM\u2010A protein expression, in parallel with analysis of JAM\u2010A gene expression data from a breast cancer clinical dataset. Our data demonstrate a novel association between JAM\u2010A gene and protein upregulation and poor prognosis in breast cancer. To mechanistically dissect this process, we used lentiviral technology to stably knock down JAM\u2010A gene expression by shRNA in MCF7 breast cancer cells, which express high\u2010endogenous levels of JAM\u2010A. We also antagonized JAM\u2010A function in wild\u2010type MCF7 cells using an inhibitory antibody that blocks JAM\u2010A dimerization. Knockdown or functional antagonism of JAM\u2010A decreased breast cancer cell migration in scratch\u2010wound assays. Reductions in \u03b21\u2010integrin protein levels were observed after JAM\u2010A\u2010knockdown in MCF7 cells, suggesting a mechanism for reduced motility after loss of JAM\u2010A. Consistent with this hypothesis, tissue microarray analysis of \u03b21\u2010integrin protein expression in invasive breast cancer tissues revealed a trend toward high \u03b21\u2010integrin protein levels being indicative of poor prognosis. Twenty\u2010two percent of patients were observed to coexpress high levels of JAM\u2010A and \u03b21\u2010integrin protein, and MDA\u2010MB\u2010231 breast cells stably overexpressing JAM\u2010A showed an increase in \u03b21\u2010integrin protein expression. Our results are consistent with a previously unreported role for JAM\u2010A overexpression as a possible mechanism contributing to progression in primary breast cancer; and a potential therapeutic target. \u00a9 2009 UICC<\/jats:p>","DOI":"10.1002\/ijc.24498","type":"journal-article","created":{"date-parts":[[2009,4,7]],"date-time":"2009-04-07T13:57:20Z","timestamp":1239112640000},"page":"1343-1351","source":"Crossref","is-referenced-by-count":120,"title":["JAM\u2010A expression positively correlates with poor prognosis in breast cancer patients"],"prefix":"10.1002","volume":"125","author":[{"given":"Elaine A.","family":"McSherry","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon F.","family":"McGee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Jirstrom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma M.","family":"Doyle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donal J.","family":"Brennan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Goran","family":"Landberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter A.","family":"Dervan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann M.","family":"Hopkins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William M.","family":"Gallagher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,7,14]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00558.2003"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65691-X"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206199"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.06.040188.002121"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2005.01.005"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.8.2600.008k28_2600_2609"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.142.1.117"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni755"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-04-1114"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2000.279.6.C1733"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M412650200"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00231-8"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.01661"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-07-3057"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa021967"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.137.1.231"},{"key":"e_1_2_6_18_2","first-page":"747","article-title":"Intestinal epithelial restitution. 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